In vitro susceptibility to antibiotics of staphylococci in biofilms isolated from orthopaedic infections
In vitro susceptibility to antibiotics of staphylococci in biofilms isolated from orthopaedic infections
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DOI：
10.1016/j.ijantimicag.2013.02.018
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发表时间：
2013-06-01
影响因子：
10.8
通讯作者：
Esteban, Jaime
中科院分区：
文献类型：
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作者：
Molina-Manso, Diana;del Prado, Gema;Esteban, Jaime
假体关节感染（PJI）与生物膜的形成有关，主要由金黄色葡萄球菌和表皮葡萄球菌引起。通常根据常规药敏试验选择治疗方法，但这些数据可能不足以检测生物膜中真正的抗生素敏感性。总共，从 PJI 患者中分离出 32 种临床菌株（17 种金黄色葡萄球菌和 15 种表皮葡萄球菌）以及 2 种收集菌株（金黄色葡萄球菌 15981 和表皮葡萄球菌 ATCC 35984），针对治疗 PJI 常用的九种抗生素（利福平、万古霉素、替加环素、克林霉素、使用卡尔加里生物膜装置（甲氧苄啶/磺胺甲恶唑、环丙沙星、氯唑西林、达托霉素和磷霉素）。没有一种抗生素被证明对这两个物种的生物膜完全有效，最低生物膜根除浓度 (MBEC) 远高于大多数抗生素的最低抑制浓度 (> 1024 mg/L)。利福平和替加环素显示 MBEC 略低，主要针对表皮葡萄球菌生物膜，并且这种葡萄球菌中只有两种菌株对几乎所有测试的抗生素敏感。这些结果表明，寻找具有抗菌和抗生物膜特性的新化合物以及开发其他可能预防和治疗 PJI 的策略是强制性的。此外，需要更多的研究来更好地了解生物膜抗菌素耐药性的机制。 (C) 2013 Elsevier B.V. 和国际化疗协会。版权所有。
Prosthetic joint infections (PJIs) are related to the formation of biofilms, mainly by Staphylococcus aureus and Staphylococcus epidermidis. Therapy is usually selected according conventional susceptibility testing, but these data may be insufficient to detect the true antibiotic susceptibility in the biofilm. In total, 32 clinical strains (17 S. aureus and 15 S. epidermidis) isolated from patients with PJIs as well as 2 collection strains (S. aureus 15981 and S. epidermidis ATCC 35984) were tested against nine antibiotics commonly used in the treatment of PJIs (rifampicin, vancomycin, tigecycline, clindamycin, trimethoprim/sulfamethoxazole, ciprofloxacin, cloxacillin, daptomycin and fosfomycin) using the Calgary Biofilm Device. None of the antibiotics proved to be totally effective against biofilms in both species, with minimum biofilm eradication concentrations (MBECs) highly above the minimum inhibitory concentrations for most of the antibiotics (> 1024 mg/L). Rifampicin and tigecycline showed MBECs slightly lower, mainly against S. epidermidis biofilms, and only two strains of this staphylococcal species were susceptible to almost all of the antibiotics tested. These results show that the search for new compounds with antimicrobial and antibiofilm properties is mandatory as well as the development of other strategies that could lead to the prevention and treatment of PJIs. In addition, more studies are necessary to obtain a better understanding of the mechanisms involved in antimicrobial resistance of biofilms. (C) 2013 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.