The Effectiveness of a Dark Septate Endophytic Fungus, Cladophialophora chaetospira SK51, to Mitigate Strawberry Fusarium Wilt Disease and With Growth Promotion Activities
The Effectiveness of a Dark Septate Endophytic Fungus, Cladophialophora chaetospira SK51, to Mitigate Strawberry Fusarium Wilt Disease and With Growth Promotion Activities
复制标题
DOI：
10.3389/fmicb.2020.00585
复制
发表时间：
2020-04-15
影响因子：
5.2
通讯作者：
Narisawa, Kazuhiko
中科院分区：
文献类型：
--
作者：
Harsonowati, Wiwiek;Marian, Malek;Narisawa, Kazuhiko
草莓镰刀菌枯萎病是由剧毒真菌尖孢镰刀菌 (Fof) 引起的，是一种毁灭性的土传疾病，在全世界（包括日本）造成严重的生产损失。 Fof是威胁全球作物安全的十大真菌病原体之一，目前尚未找到有效控制该病原体的方法。本研究旨在研究深色有隔内生真菌 (DSE) 对 Fof 的有效性，以开发一种高效、有效且环保的方法来改善植物健康和适应性。总共评估了 19 种真菌分离株，其中 3 种（SK47、SK48 和 SK51）是根据其在使用土壤系统的受控生长室条件下抑制疾病的有效性而选择的。分离株 SK47、SK48 和 SK51 分别将疾病严重程度抑制了 85.71%、61.90 和 90.48%。基于高度保守的小亚基 (SSU)、内转录间隔区 (ITS) 和大亚基 (LSU) nrRNA 区域的分子鉴定将这些分离物分别鉴定为 DSE Exophiala sp.、Exophiala pisciphila 和 Cladophialophora chaetospira。这些序列以登录号保存在 GenBank 数据库中。值得注意的是，我们的结果表明，与对照植物和其他两种候选真菌相比，分离物 C.chaetospira SK51 通过显着增加植物生长参数（芽和根干质量、叶绿素含量、花芽萌发和果实数量）而具有优异的生长促进活性和疾病抑制作用。 C.chaetospira SK51 的根部定植被可视化，并证实与草莓植物的共生成功发生。我们的研究结果为 DSE 真菌 C. chaetospira SK51 作为草莓植物生物防治剂的应用提供了新的见解，可以促进植物生长、花芽萌发和果实形成。 C. chaetospira SK51 对宿主植物表现出显着的有益特性，它有可能用于开发新的、安全且有效的处理方法，作为化肥和杀菌剂的替代品，实现可持续作物保护。
Strawberry Fusarium wilt, caused by the virulent fungus Fusarium oxysporum formae speciales fragariae (Fof) is a devastating soil-borne disease that causes severe production losses worldwide, including Japan. Fof is one of the top 10 fungal pathogens that threaten global crop security, and a method to effectively control this pathogen has yet to be found. This study aimed to investigate the effectiveness of dark septate endophytic (DSE) fungi against Fof to develop an efficient, effective, and environmentally friendly approach to improve plant health and fitness. A total of 19 fungal isolates were assessed, out of which three (SK47, SK48, and SK51) were selected based on their effectiveness in disease suppression in controlled growth chamber conditions using a soil system. Isolates SK47, SK48, and SK51 suppressed disease severity by 85.71, 61.90, and 90.48%, respectively. Molecular identification based on highly conserved small subunit (SSU), internal transcribed spacer (ITS), and large subunit (LSU) nrRNA regions identified these isolates as DSE Exophiala sp., Exophiala pisciphila, and Cladophialophora chaetospira, respectively. The sequences were deposited under accession numbers - in the GenBank database. Notably, our results revealed that isolate C. chaetospira SK51 possessed superior growth promotion activities as well as disease suppression by significantly increased plant growth parameters (shoot and root dry mass, chlorophyll content, flower bud initiation, and number of fruit) in comparison to control plants and other two fungal candidates. Root colonization by C. chaetospira SK51 was visualized, and it was confirmed that the symbiosis with strawberry plants occurred successfully. Our results provide new insights in the application of DSE fungus C. chaetospira SK51 as a biocontrol agent on strawberry plants could promote plant growth, flower bud initiation, and fruit formation. C. chaetospira SK51 exhibited remarkable beneficial traits for the host plant, and it can potentially be applied in the development of new, safe, and effective treatments as an alternative to chemical fertilizers and fungicides for sustainable crop protection.