A global view of gene activity at the flowering transition phase in precocious trifoliate orange and its wild-type [Poncirus trifoliata (L.) Raf.] by transcriptome and proteome analysis
A global view of gene activity at the flowering transition phase in precocious trifoliate orange and its wild-type [Poncirus trifoliata (L.) Raf.] by transcriptome and proteome analysis
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通过转录组和蛋白质组分析全面了解早熟枳及其野生型[Poncirus trifoliata (L.) Raf.]开花过渡期的基因活性
DOI：
10.1016/j.gene.2012.07.090
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发表时间：
2012-11-15
期刊：
影响因子：
3.5
通讯作者：
Zhang, Jin-Zhi
中科院分区：
文献类型：
--
作者：
Ai, Xiao-Yan;Lin, Guang;Zhang, Jin-Zhi
关于开花时间调控的分子遗传学，我们所知道的大部分来自于对模式植物的研究。然而，与模式植物相比，对多年生植物或具有特殊生长习性的物种的开花过渡调控知之甚少。本文对早花三叶橙突变体(早熟三叶橙)及其野生型春梢进行了比较转录组和蛋白质组分析。通过RNA-Seq分析，在两种类型中共观察到19,215个读图基因。其中，1450个基因和1159个基因分别在突变型文库和野生型文库中被特异性观察到。有355个基因在两种基因型中有不同的表达。通过iTRAQ技术鉴定了总共1664个蛋白质，在50%的比较中，转录和蛋白质谱在整个时间过程中是平行的，但也观察到了不同的模式，表明转录后事件。此外，对信使RNA剪接事件的全球调查在12,688个基因中发现了16,343个剪接连接，并表明替代3‘剪接是最普遍的替代剪接形式。我们进一步鉴定了5698个新的转录区域，这些区域在两个基因类型中与注释的柑橘转录组不重叠。了解柑橘开花转换的调控机制有助于开发新的遗传或管理策略以提高果实产量。(C)2012爱思唯尔B.V.保留所有权利。
Most of what we know about the molecular genetics of flowering time regulation comes from studies in the model plants. However, little is known about the regulation of flowering transition in perennial species or in species with particular growth habits compared with model plants. Here comparative transcriptome and proteome profiling of spring shoots was performed on an early flowering trifoliate orange mutant (precocious trifoliate orange, Poncirus trifoliate) and its wild-type. A total of 19,215 read-mapped genes were observed in two genotypes by RNA-Seq. Of these, 1450 and 1159 genes were specifically observed in the mutant and wild-type libraries, respectively. There were 355 genes that were expressed differently in the two genotypes. A total of 1664 proteins were identified by the iTRAQ technique, and transcript and protein profiles were parallel across the time course for 50% of the comparisons made, but divergent patterns were also observed, indicative of post-transcriptional events. In addition, a global survey of messenger RNA splicing events identified 16,343 splice junctions among 12,688 genes and showed that alternative 3' splice is the most prevalent form of alternative splicing. We further identify 5698 novel transcribed regions that are not overlapping with annotated citrus transcriptome in two genotypes. Understanding of the regulation of flowering transition in citrus can help in the development of new genetic or management strategies to improve fruit production. (C) 2012 Elsevier B.V. All rights reserved.