Katanin, a microtubule-severing protein, is a novel AAA ATPase that targets to the centrosome using a WD40-containing subunit
Katanin, a microtubule-severing protein, is a novel AAA ATPase that targets to the centrosome using a WD40-containing subunit
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DOI：
10.1016/s0092-8674(00)81578-0
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发表时间：
1998-04-17
期刊：
影响因子：
64.5
通讯作者：
McNally, FJ
中科院分区：
文献类型：
--
作者：
Hartman, JJ;Mahr, J;McNally, FJ
微管在中心体的解体参与有丝分裂纺锤体的功能。微管切断蛋白katanin，异二聚体的60和80 kDa的亚基，先前纯化，并显示本地化的中心体在体内。在这里，我们报告的katanin亚基的序列和活动，p60是AAA家族的ATP酶的一个新成员，我们表明，表达的p60具有微管刺激的ATP酶和微管切断活动的情况下，p80。p80是一种新的含有WD 40重复序列的蛋白质，其经常参与蛋白质-蛋白质相互作用。p80 WD 40结构域不参与p60二聚化，但定位于转染的哺乳动物细胞中的中心体。这些结果表明katanin的活性被分离成具有酶活性的亚基（p60）和将酶靶向中心体的亚基（p80）。
Microtubule disassembly at centrosomes is involved in mitotic spindle function. The microtubule-severing protein katanin, a heterodimer of 60 and 80 kDa subunits, was previously purified and shown to localize to centrosomes in vivo. Here we report the sequences and activities of the katanin subunits, p60 is a new member of the AAA family of ATPases, and we show that expressed p60 has microtubule-stimulated ATPase and microtubule-severing activities in the absence of p80. p80 is a novel protein containing WD40 repeats, which are frequently involved in protein-protein interactions. The p80 WD40 domain does not participate in p60 dimerization, but localizes to centrosomes in transfected mammalian cells. These results indicate katanin's activities are segregated into a subunit (p60) that possesses enzymatic activity and a subunit (p80) that targets the enzyme to the centrosome.