Biodegradation of Phenanthrene in Polycyclic Aromatic Hydrocarbon-Contaminated Wastewater Revealed by Coupling Cultivation-Dependent and -Independent Approaches
Biodegradation of Phenanthrene in Polycyclic Aromatic Hydrocarbon-Contaminated Wastewater Revealed by Coupling Cultivation-Dependent and -Independent Approaches
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通过耦合依赖于培养的方法和独立于培养的方法揭示了多环芳烃污染废水中菲的生物降解
DOI：
10.1021/acs.est.6b04366
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发表时间：
2017-03-21
影响因子：
11.4
通讯作者：
Zhang, Gan
中科院分区：
文献类型：
--
作者：
Li, Jibing;Luo, Chunling;Zhang, Gan
采用DNA稳定同位素探针法(DNA-SIP)对多环芳烃(PAHs)污染废水中降解菲(Phe)的本地微生物进行了鉴定。除了著名的苯丙氨酸降解菌不动杆菌和狮身人面像外，首次发现Kouleothrix和Sandaracinbacter对本土苯丙氨酸的生物降解有直接作用。此外，还发现了一种新的苯丙氨酸降解菌--坦度不动杆菌。经DNA-SIP和直接培养鉴定为LJ-5。这是多环芳烃污染水的生物修复过程中首次报道和参考坦度艾美耳氏菌。在C-13处理的重组份中检测到与Phe代谢有关的PAH-RHDα基因，但在唐氏沼虾LJ-5中未能扩增出PAH-RHDα基因。相反，该菌株含有儿茶酚1，2-双加氧酶和原儿茶酸3，4-双加氧酶的α/β亚基，表明利用-β-酮己二酸途径降解苯丙氨酸和相关的芳香族化合物。这些发现通过结合依赖栽培和不依赖栽培的方法，增加了我们目前关于降解苯丙氨酸的微生物的知识，并提供了对土著苯丙氨酸降解群落多样性的更深层次的洞察。
The indigenous microorganisms responsible for degrading phenanthrene (PHE) in polycyclic aromatic hydrocarbons (PAHs)-contaminated wastewater were identified by DNA-based stable isotope probing (DNA-SIP). In addition to the well-known PHE degraders Acinetobacter and Sphingobium, Kouleothrix and Sandaracinobacter were found, for the first time, to be directly responsible for indigenous PHE biodegradation. Additionally, a novel PHE degrader, Acinetobacter tandoii sp. LJ-5, was identified by DNA-SIP and direct cultivation. This is the first report and reference to A. tandoii involved in the bioremediation of PAHs-contaminated water. A PAH-RHD alpha gene involved in PHE metabolism was detected in the heavy fraction of C-13 treatment, but the amplification of PAH-RHD alpha gene failed in A. tandoii LJ-5. Instead, the strain contained eatechol 1,2-dioxygenase and alpha/beta subunits of protocatechuate 3,4-dioxygenase, indicating use of the-beta-ketoadipate pathway to degrade PHE and related aromatic compounds. These findings add to our current knowledge on microorganisms degrading PHE by combining cultivation-dependent and cultivation-independent approaches and provide deeper insight into the diversity of indigenous PHE-degrading communities.