Identification of two novel markers for alveolar epithelial type I and II cells
Identification of two novel markers for alveolar epithelial type I and II cells
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DOI：
10.1016/j.bbrc.2004.05.048
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发表时间：
2004-07-02
影响因子：
3.1
通讯作者：
Liu, L
中科院分区：
文献类型：
--
作者：
Chen, ZM;Jin, NL;Liu, L
肺泡上皮I型和II型细胞（AEC I和II）在肺泡表面紧密排列。为了分离和评估这两种细胞的功能和其他特性，对AEC I和II的精确鉴定非常感兴趣。本研究以AEC Ⅱ体外转分化为AEC Ⅰ样细胞为模型，利用DNA微阵列技术鉴定AEC Ⅰ和AEC Ⅱ细胞特异性标志物。实时定量PCR证实了5个AEC I基因：成纤维细胞生长因子受体激活蛋白1、水通道蛋白5、嘌呤能受体P2 X 7（M7）、干扰素诱导蛋白和Bcl 2相关蛋白，以及1个AEC II基因：γ-氨基丁酸受体pi亚基（GABRP）。对培养细胞和大鼠肺组织的免疫染色表明，GABRP和P2 X7蛋白分别在AEC II和AEC I中特异性表达。大鼠肺组织原位杂交证实GABRPmRNA定位于II型细胞。本研究中鉴定的P2 X7和GABRP可作为潜在的AEC I和AEC II标记物用于研究肺上皮细胞生物学和监测肺损伤。(C)2004爱思唯尔公司All rights reserved.
Alveolar epithelial type I and type II cells (AEC I and II) are closely aligned in alveolar surface. There is much interest in the precise identification of AEC I and II in order to separate and evaluate functional and other properties of these two cells. This study aims to identify specific AEC I and AEC II cell markers by DNA microarray using the in vitro trans-differentiation of AEC II into AEC I-like cells as a model. Quantitative real-time PCR confirmed five AEC I genes: fibroblast growth factor receptor-activating protein 1, aquaporin 5, purinergic receptor P2X 7 (M7), interferon-induced protein, and Bcl2-associated protein, and one AEC II gene: gamma-aminobutyric acid receptor pi subunit (GABRP). Immunostaining on cultured cells and rat lung tissue indicated that GABRP and P2X7 proteins were specifically expressed in AEC II and AEC I, respectively. In situ hybridization of rat lung tissue confirmed the localization of GABRP mRNA in type II cells. P2X7 and GABRP identified in this study could be used as potential AEC I and AEC II markers for studying lung epithelial cell biology and monitoring lung injury. (C) 2004 Elsevier Inc. All rights reserved.