Evaluation of a one-step test for rapid, in practice detection of rotavirus in farm animals

Evaluation of a one-step test for rapid, in practice detection of rotavirus in farm animals
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DOI:
10.1136/vr.138.16.393
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发表时间:
1996-04-20
期刊:
影响因子:
2.2
通讯作者:
Esfandiari, J
Esfandiari, J
中科院分区:
农林科学3区
文献类型:
--
作者:
Klingenberg, KD;Esfandiari, J

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通过对161份牛、猪和马的粪便样本进行常规检测,对检测A组轮状病毒的免疫层析试验与A组轮状病毒的参考酶联免疫吸附试验进行了评估。与酶联免疫吸附试验相比,该方法的敏感性为89%,特异性为99%。其重现性为100%。该测试方法简单、快速,适合于实际应用。
An immunochromatographic test for the detection of group A rotavirus was evaluated against a reference group A rotavirus ELISA, by using a panel of 161 bovine, porcine and equine faecal samples submitted for routine examination. The sensitivity of the test was 89 per cent and the specificity 99 per cent compared with the ELISA. Its reproducibility was 100 per cent. The simplicity and rapidity of the test procedure make it suitable for use in practice.