The long-term oral administration of a product derived from a probiotic, Clostridium butyricum induced no pathological effects in rats.
The long-term oral administration of a product derived from a probiotic, Clostridium butyricum induced no pathological effects in rats.
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DOI：
10.3892/ijmm.12.4.571
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发表时间：
2002-02
影响因子：
5.4
通讯作者：
Y. Araki;A. Andoh;J. Takizawa;W. Takizawa;Y. Fujiyama
中科院分区：
文献类型：
--
作者：
Y. Araki;A. Andoh;J. Takizawa;W. Takizawa;Y. Fujiyama
最近的研究表明，短链脂肪酸（SCFAs）对一些人类和实验动物疾病具有治疗作用。在我们之前的研究中，我们表明丁酸梭菌在所使用的培养系统中产生高水平的SCFAs。此外，基于酸奶的添加剂可有效消除和掩盖产品中源自这些SCFA的气味。最近，我们报告了经口给予高浓度（50% w/w）的丁酸梭菌产品17天，未引起大鼠病理学异常。本研究的目的是研究该产品在大鼠中长期经口给药的影响。对5周龄的雄性和雌性Wistar汉诺威GALAS大鼠给予标准饮食加上来自丁酸梭菌的产物（5%w/w）和0.1%添加剂的混合物16个月（n=6）。对照大鼠允许相同的标准饮食加自来水（5%w/w）与0.1%的添加剂（n=6）。16个月后，进行了剖腹手术。随后进行血细胞计数、生化和电解质分析。对食管、胃、小肠、大肠和胰腺进行了肉眼和显微镜检查。结果表明，大鼠在实验期间生长正常。产品喂养大鼠的体重与对照喂养大鼠的体重相当。除了喂食产品的雄性大鼠的大肠重量显著增加外，喂食产品的大鼠和喂食对照品的大鼠之间的器官重量无显著差异。血细胞计数、生化和电解质分析或肉眼和显微镜检查结果均未发现病理学异常。
Recent studies have suggested that short chain fatty acids (SCFAs) exert a therapeutic effect on some human and experimental animal diseases. In our previous study, we showed that Clostridium butyricum produces high levels of SCFAs in the culture system used. In addition, an additive based on yogurt was effective in eliminating and masking the odor derived from these SCFAs in the product. Recently, we reported that the oral administration of a high concentration (50% w/w) of this product derived from Clostridium butyricum for 17 days caused no pathological abnormalities in rats. The aim of the present study was to investigate the effects of the prolonged oral administration of this product in rats. Male and female Wistar Hannover GALAS rats, 5 weeks old, were given a mixture of a standard diet plus the product derived from Clostridium butyricum (5% w/w) with 0.1% additive for 16 months (n=6). The control rats were allowed the same standard diet plus tap water (5% w/w) with 0.1% additive (n=6). After 16 months, a laparotomy was performed. A hemocyte count, and biochemical and electrolyte analyses were subsequently carried out. The esophagus, stomach, small intestine, large intestine and pancreas were investigated macroscopically and microscopically. The results showed that the rats grew normally for the duration of the experimental period. The body weights of the product-fed rats were comparable with those of the control-fed rats. There were no significant differences in the organ weight between the product- and control-fed rats, except for a significantly increased weight of the large intestine in the product-fed male rats. No pathological abnormalities were found in the hemocyte count, the biochemical and electrolyte analyses, or the macroscopic and microscopic findings.