Structural characterization of virion proteins and genomic RNA of human parainfluenza virus 3
Structural characterization of virion proteins and genomic RNA of human parainfluenza virus 3
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人副流感病毒3病毒颗粒蛋白和基因组RNA的结构表征
作者：
D. Storey;K. Dimock;C. Kang
研究了人副流感病毒3型的病毒粒子蛋白和基因组RNA。病毒粒子含有七种主要蛋白质和两种次要蛋白质。三个195 × 10(3)分子量（195K）、87K和67K的蛋白与病毒粒子的核衣壳相关，分别被命名为L、P和NP。三种蛋白可以用[14C]氨基葡萄糖标记，分子量分别为69K、60K和46K。我们将这些蛋白分别命名为HN、F0和F1。HN蛋白具有链间二硫键，但不参与二硫键形成同聚体。F1似乎来源于一个复合体，F1,2，它在非还原条件下具有与F0相似的电泳迁移率。35K的蛋白质与病毒粒子的包膜成分有关，并在低盐条件下聚集；该蛋白被命名为M.人副流感病毒3号的基因组是一个线性RNA分子，分子量约为4.6 X 10(6)。
The virion proteins and genomic RNA of human parainfluenza virus 3 have been characterized. The virion contains seven major and two minor proteins. Three proteins of 195 X 10(3) molecular weight (195K), 87K, and 67K are associated with the nucleocapsid of the virion and have been designated L, P, and NP, respectively. Three proteins can be labeled with [14C]glucosamine and have molecular weights of 69K, 60K, and 46K. We have designated these proteins as HN, F0, and F1, respectively. HN protein has interchain disulfide bonds, but does not participate in disulfide bonding to form homomultimeric forms. F1 appears to be derived from a complex, F1,2, that has an electrophoretic mobility similar to that of F0 under nonreducing conditions. A protein of 35K is associated with the envelope components of the virion and aggregates under low-salt conditions; this protein has been designated M. The genome of human parainfluenza virus 3 is a linear RNA molecule with a molecular weight of approximately 4.6 X 10(6).