PEPTIDE CHAIN ELONGATION RATE AND RIBOSOMAL ACTIVITY IN SACCHAROMYCES-CEREVISIAE AS A FUNCTION OF THE GROWTH-RATE
PEPTIDE CHAIN ELONGATION RATE AND RIBOSOMAL ACTIVITY IN SACCHAROMYCES-CEREVISIAE AS A FUNCTION OF THE GROWTH-RATE
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DOI：
10.1007/bf00337800
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发表时间：
1979-01-01
期刊：
影响因子：
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通讯作者：
GULLOV, K
中科院分区：
文献类型：
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作者：
BONVEN, B;GULLOV, K
采用Gausing（1972）和Young and Bremer（1976）对新生多肽和成品多肽进行放射性标记的动力学方法，估计了酿酒酵母在两种不同生长速率下的肽链延伸率。在酵母氮基培养基中，在30℃条件下，葡萄糖和乙酸对二倍体菌株的伸长率分别为9.3个氨基酸/s和5.5个氨基酸/s。C,分别。这些数据与已发表的关于核糖体效率作为生长速率函数的值一起，允许估计核糖体蛋白质的合成速率作为总蛋白质合成速率的函数。R，以及在蛋白质合成中活跃的核糖体的部分。显然在酿酒葡萄球菌中。R在很大程度上与生长速率无关，而活性核糖体的比例随着生长速率的降低而降低。
The peptide-chain elongation rate of S. cerevisiae at 2 different growth rates was estimated by the kinetics of radioactive labeling of nascent and finished polypeptides as described by Gausing, 1972, and Young and Bremer, 1976. The elongation rates of a diploid strain cultured in yeast nitrogen base supplemented with glucose or acetate were 9.3 amino acids/s and 5.5 amino acids/s at 30.degree. C, respectively. These data together with published values on the ribosomal efficiency as a function of growth rate permit estimation of the rate of synthesis of ribosomal proteins as a function of the rate of total protein synthesis, .alpha.r, and the fraction of ribosomes that are active in protein synthesis. Apparently in S. cerevisiae .alpha.r is largely independent of the growth rate while the fraction of active ribosomes decreases with decreasing growth rate.