Suppression Subtractive Hybridization Identified Differentially Expressed Genes in Pistil Mutations in Wheat
Suppression Subtractive Hybridization Identified Differentially Expressed Genes in Pistil Mutations in Wheat
复制标题
抑制消减杂交鉴定小麦雌蕊突变中的差异表达基因
DOI：
10.1007/s11105-010-0249-2
复制
发表时间：
2011-06-01
影响因子：
2.1
通讯作者：
Cai, Peng
中科院分区：
文献类型：
--
作者：
Yang, Zaijun;Peng, Zhengsong;Cai, Peng
为了解小麦三雌蕊突变的分子机制，利用SSH技术构建了两对小麦近等基因系川麦28三雌蕊（CMTP）和中国春三雌蕊（CSTP）的正向消减cDNA文库。CMTP系中总共有68个克隆和CSTP系中总共有197个克隆被鉴定为潜在的过表达克隆。CMTP系中68个克隆中有32个属于未知蛋白质;而其余30个克隆与参与蛋白质调节和蛋白质合成、信号转导和离子转运蛋白的不同类别的基因具有同源性。CSTP系中约67%的基因未分类或在数据库中没有匹配（“无命中”），约33%的鉴定基因编码具有已知功能的多肽。两个正向cDNA文库之间的cDNA克隆的序列比较显示，四个基因，编码硫氧还蛋白H，泛素蛋白连接酶，MCM 2，和泛喹啉-细胞色素C还原酶复合物14 kDa的蛋白质，在两个文库中过表达。这些基因可能在决定小麦突变体的三雌蕊性状中起重要作用。
To understand the molecular mechanism of the three pistils mutation in wheat, two forward subtractive cDNA libraries from two pairs of near-isogenic wheat lines, Chuanmai 28 three pistils (CMTP) and Chinese Spring three pistils (CSTP), were constructed using SSH. A total of 68 clones in CMTP lines and 197 clones in CSTP lines were identified as potentially over-expressed clones. Thirty-two out of 68 clones in CMTP lines belonged to unknown proteins; while, the remaining 30 clones shared homology to diverse classes of genes involved in protein modulation and protein synthesis, signal transduction, and ion transporters. Approximately 67% of genes in CSTP lines were either unclassified or had no matches (“no hits”) in the database and about 33% of identified genes encoded polypeptides with known functions. Sequence comparisons of cDNA clones between the two forward cDNA libraries revealed that four genes, encoding thioredoxin H, ubiquitin protein ligases, MCM2, and ubiquinol-cytochrome C reductase complex 14 kDa proteins, were over-expressed in both libraries. These genes would likely play an important role in determining the three pistils trait in the mutant wheat line.