Nitric Oxide and Protein S-Nitrosylation Are Integral to Hydrogen Peroxide-Induced Leaf Cell Death in Rice
Nitric Oxide and Protein S-Nitrosylation Are Integral to Hydrogen Peroxide-Induced Leaf Cell Death in Rice
复制标题
DOI：
10.1104/pp.111.184531
复制
发表时间：
2012-01-01
期刊：
影响因子：
7.4
通讯作者：
Chu, Chengcai
中科院分区：
文献类型：
--
作者：
Lin, Aihong;Wang, Yiqin;Chu, Chengcai
一氧化氮（NO）是一种重要的氧化还原活性小分子，参与植物生长发育的各个方面。在这里，我们报告了一个NO积累突变体，一氧化氮过量1（noe 1），在水稻（Oryza sativa），分离相应的基因，并分析其在NO介导的叶细胞死亡的作用。图位克隆显示NOE 1编码水稻过氧化氢酶OsCATC。此外，noe 1导致过氧化氢（H2 O2）在叶片中的增加，从而促进NO的产生通过激活硝酸还原酶。去除过量的NO减少细胞死亡的叶片和悬浮培养来自noe 1植物，暗示NO作为一个重要的内源性介质H2 O2诱导的叶细胞死亡。水稻S-亚硝基谷胱甘肽还原酶基因（GSNOR 1）的过表达产生的细胞内S-亚硝基硫醇（SNO）水平的降低，可调节蛋白质S-亚硝基化的整体水平，减轻noe 1植物叶细胞死亡。因此，S-亚硝基化也参与了光依赖性叶细胞死亡的noe 1。利用生物素转换测定、纳米液体色谱和串联质谱，在野生型和noe 1植物中鉴定了S-亚硝基化蛋白。仅在noe 1植物中鉴定的NO靶点包括甘油醛3-磷酸脱氢酶和硫氧还蛋白，据报道它们参与了动物中S-亚硝基化调节的细胞死亡。总的来说，我们的数据表明，NO和SNOs是H2 O2诱导水稻叶细胞死亡过程中的重要介质。
Nitric oxide (NO) is a key redox-active, small molecule involved in various aspects of plant growth and development. Here, we report the identification of an NO accumulation mutant, nitric oxide excess1 (noe1), in rice (Oryza sativa), the isolation of the corresponding gene, and the analysis of its role in NO-mediated leaf cell death. Map-based cloning revealed that NOE1 encoded a rice catalase, OsCATC. Furthermore, noe1 resulted in an increase of hydrogen peroxide (H2O2) in the leaves, which consequently promoted NO production via the activation of nitrate reductase. The removal of excess NO reduced cell death in both leaves and suspension cultures derived from noe1 plants, implicating NO as an important endogenous mediator of H2O2-induced leaf cell death. Reduction of intracellular S-nitrosothiol (SNO) levels, generated by overexpression of rice S-nitrosoglutathione reductase gene (GSNOR1), which regulates global levels of protein S-nitrosylation, alleviated leaf cell death in noe1 plants. Thus, S-nitrosylation was also involved in light-dependent leaf cell death in noe1. Utilizing the biotin-switch assay, nanoliquid chromatography, and tandem mass spectrometry, S-nitrosylated proteins were identified in both wild-type and noe1 plants. NO targets identified only in noe1 plants included glyceraldehyde 3-phosphate dehydrogenase and thioredoxin, which have been reported to be involved in S-nitrosylation-regulated cell death in animals. Collectively, our data suggest that both NO and SNOs are important mediators in the process of H2O2-induced leaf cell death in rice.