Physical crosslinking of collagen fibers: comparison of ultraviolet irradiation and dehydrothermal treatment.
Physical crosslinking of collagen fibers: comparison of ultraviolet irradiation and dehydrothermal treatment.
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DOI：
10.1002/jbm.820291108
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发表时间：
1995-11
期刊：
影响因子：
--
通讯作者：
K. Weadock;E. J. Miller;Lisa D. Bellincampi;J. Zawadsky;M. Dunn
中科院分区：
文献类型：
--
作者：
K. Weadock;E. J. Miller;Lisa D. Bellincampi;J. Zawadsky;M. Dunn
胶原性生物材料的强度、吸收率和生物相容性受到交联剂的方法和程度的深刻影响。我们比较了两种物理交联方法--紫外线照射(UV)(254 Nm)和脱水热(DHT)处理对从酸性分散的牛真皮I型胶原蛋白挤出的胶原纤维的力学性能和分子完整性的影响。经紫外线照射15min的胶原纤维的极限拉伸强度(54 Mpa)和弹性模量值(184 Mpa)大于或等于DHT处理3或5天的纤维的值。紫外线照射是一种快速且易于控制的增加胶原纤维机械强度的方法。用稀醋酸和有限胃酶消化从交联样品中提取的胶原的表征表明，紫外线和DHT处理都会导致至少一部分胶原分子的碎裂。天然胶原结构的部分丧失可能会影响细胞在胶原纤维类似物上的附着、迁移和增殖。这些问题目前正在我们的实验室解决。
The strength, resorption rate, and biocompatibility of collagenous biomaterials are profoundly influenced by the method and extent of crosslinking. We compared the effects of two physical crosslinking methods, ultraviolet irradiation (UV) (254 nm) and dehydrothermal (DHT) treatment, on the mechanical properties and molecular integrity of collagen fibers extruded from an acidic dispersion of type I bovine dermal collagen. Collagen fibers exposed to UV irradiation for 15 min had ultimate tensile strength (54 MPa) and modulus (184 MPa) values greater than or equivalent to values for fibers crosslinked with DHT treatment for 3 or 5 days. UV irradiation is a rapid and easily controlled means of increasing the mechanical strength of collagen fibers. Characterization of collagen extracted from the crosslinked samples by dilute acetic acid and limited pepsin digestion indicate that both UV and DHT treatments cause fragmentation of at least a portion of the collagen molecules. Partial loss of the native collagen structure may influence attachment migration, and proliferation of cells on collagen fiberbased ligament analogs. These issues are currently being addressed in our laboratory.