The ectopic expression of PttKN1 gene causes pleiotropic alternation of morphology in transgenic carnation (Dianthus caryophyllus L.)
The ectopic expression of PttKN1 gene causes pleiotropic alternation of morphology in transgenic carnation (Dianthus caryophyllus L.)
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DOI：
10.1007/s11738-009-0334-z
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发表时间：
2009-06
影响因子：
2.6
通讯作者：
Lai-Sheng Meng;Jiang-Ping Song;Shaoshuang Sun;Chong Wang
中科院分区：
文献类型：
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作者：
Lai-Sheng Meng;Jiang-Ping Song;Shaoshuang Sun;Chong Wang
康乃馨（Dianthus carpellusL.）是世界上最重要的观赏植物之一。香石竹的形态改造对香石竹的商业价值具有重要意义，但目前尚未见相关报道，从杂种白杨维管形成层中分离到的PttKN 1（Populus tremula×Tremuoides knotted 1）是KNOX基因家族的新成员。本研究利用农杆菌介导法将35 S：PttKN 1转化香石竹。将随后获得的所有初级转化体置于表型类别中并自花授粉。共获得32个具有异常表型的T0后代。PCR检测证明了转基因植株的有效性。3235 S：PttKN 1植株的表型与野生型植株不同，表现为：（1）叶序改变（15/32）：野生型植株具有典型的对生叶序，而转基因植株具有三对生和多对生叶序。（2）茎的变异（9/32）：野生型植株的茎为圆形，但部分转基因植株的茎变粗、变扁;（3）香石竹转基因植株（8/32）全部变矮。这些形态变异表明我们已经成功地获得了一些新的香石竹品系。这些线可以具有潜在的实际应用。最后，对稳定遗传系的选择进行了讨论。
Carnation (Dianthus caryophyllusL.) is one of the most important ornamental plants in the world. Though morphological modification of carnation is very important to its commercial value, there have been no relevant reports until now.PttKN1(Populus tremula×Tremuoides knotted1), isolated from the vascular cambial region of hybrid aspen, is a novel member ofKNOXgene family. In this paper, we transformed35S:PttKN1to carnation viaAgrobacterium tumefaciens. All primary transformants subsequently obtained were placed into phenotypic categories and self-pollinated. A total of 32 T0 progeny with aberrant phenotypes were obtained. PCR assay proved the validity of these transgenic plants. Phenotypes of 3235S:PttKN1plants were distinct from those of wild-type plants, including: (1) modification of phyllotaxis (15/32): wild-type carnation was with typical opposite phyllotaxis, while transgenic plants displayed tricussate whorled and multiple-cussate whorled phyllotaxis. Irregular modification of phyllotaxis was also observed; (2) modification of stem (9/32): wild-type stems were round; however, some transgenic plants exhibited much thicker and flatter stem; (3) the whole transgenic plants of carnation (8/32) became dwarf. These morphological modifications of carnation indicate that we have successfully attained some novel lines of carnation. These lines can have potential practical applications. In conclusion, the selection of stably genetic lines is discussed.