THE HEMATOGENOUS ORIGIN OF OSTEOCLASTS - EXPERIMENTAL-EVIDENCE FROM OSTEOPETROTIC (MICROPHTHALMIC) MICE TREATED WITH SPLEEN-CELLS FROM BEIGE MOUSE DONORS
THE HEMATOGENOUS ORIGIN OF OSTEOCLASTS - EXPERIMENTAL-EVIDENCE FROM OSTEOPETROTIC (MICROPHTHALMIC) MICE TREATED WITH SPLEEN-CELLS FROM BEIGE MOUSE DONORS
复制标题
DOI：
10.1002/aja.1001610102
复制
发表时间：
1981-01-01
影响因子：
--
通讯作者：
WALKER, DG
中科院分区：
文献类型：
--
作者：
MARKS, SC;WALKER, DG
小眼（mi）小鼠的骨硬化症特征性的骨骼质量过大和骨吸收减少可以通过照射和移植正常同窝小鼠的脾细胞来纠正。米色（bg）小鼠中的破骨细胞，一种没有骨硬化症的突变，具有巨大的溶酶体颗粒。这两个事实被用来追踪破骨细胞谱系。用全身照射和来自米色供体的脾细胞处理的小眼小鼠吸收了过多的骨骼质量，并从骨硬化症中恢复。经处理的mi小鼠中的破骨细胞具有巨大的溶酶体颗粒，并且通过光镜和透射电镜检查时与bg供体中发现的颗粒相似。显然，哺乳动物破骨细胞是血原性的。
Excessive skeletal mass and reduced bone resorption characteristic of osteopetrosis in microphthalmic (mi) mice can be corrected by irradiation and transfer of spleen cells from a normal littermate. Osteoclasts in beige (bg) mice, a mutation without osteopetrosis, have giant lysosomal granules. These 2 facts were exploited to trace osteoclast lineage. Microphthalmic mice treated with whole body irradiation and spleen cells from a beige donor resorbed the excessive skeletal mass and recovered from osteopetrosis. Osteoclasts in treated mi mice had giant lysosomal granules and resembled those found in bg donors when examined by light and transmission electron microscopy. Apparently, mammalian osteoclasts are of hematogenous origin.