Measurement of inducible proteins improves the precision of the local pigeon crop-sac bioassay for prolactin.
Measurement of inducible proteins improves the precision of the local pigeon crop-sac bioassay for prolactin.
复制标题
诱导蛋白的测量提高了当地鸽子嗉囊生物测定催乳素的精度。
DOI：
10.1016/0024-3205(92)90532-t
复制
发表时间：
1992
期刊：
影响因子：
6.1
通讯作者：
Nicoll,CS
中科院分区：
文献类型：
--
作者：
Lebovic,DI;Nicoll,CS
鸽子嗉囊是众所周知的催乳素（PRL）靶器官。 Pukac 和 Horseman (Endocrinology 114: 1718, 1984) 报告称，注射激素会导致该器官中几种特定蛋白质的表达发生变化。我们研究了测量其中三种蛋白质的含量可能会提高当地鸽子嗉囊囊测定 PRL 的精度的可能性。两天内给鸽子注射四次磷酸盐缓冲盐水或不同剂量的羊（o）PRL，并在第三天处死。测量鸽子嗉囊粘膜上皮细胞的DNA和总蛋白含量，并通过SDS-PAGE处理匀浆上皮细胞的上清液。通过光密度测定法测量染色凝胶中三种特定蛋白质的量。 oPRL 处理增加了嗉囊粘膜组织中其中两种蛋白质（CP 14 和 CP 25）的浓度，并降低了第三种蛋白质（CP 17）的浓度。总蛋白或单个蛋白条带变化的测量给出了精度较差的剂量反应关系（λ>0.3）。 DNA 含量的测量给出了具有相当好的 λ 值 (0.16) 的测定终点，但当 CP 14 和 CP 25 的量变化表示为 CP 17 中发生的变化的比率时，获得了非常精确的测定终点 (λ=0.06−0.08)。
The pigeon crop-sac is a well-known target organ of prolactin (PRL). Pukac and Horseman (Endocrinology 114: 1718, 1984) reported that injections of the hormone caused changes in the expression of several specific proteins in that organ. We investigated the possibility that measuring the amounts of three of these proteins might improve the precision of the local pigeon crop-sac assay to PRL. Pigeons were given four injections of phosphate-buffered saline or different doses of ovine (o) PRL for 2 days and were killed on day 3. The DNA and total protein content of their crop-sac mucosal epithelial cells were measured and a supernatant of homogenized epithelial cells was processed by SDS-PAGE. The amounts of the three specific proteins in the stained gels were measured by densitometry. Treatment with oPRL increased the concentration of two of these proteins in the crop mucosal tissue (CP 14 and CP 25) and decreased that of the third one (CP 17). Measurement of total protein or of changes in the individual protein bands gave dose-response relationships with poor indices of precision (λ>0.3). Measurement of DNA content gave an assay endpoint with a reasonably good λ value (0.16) but when the changes in the amounts of CP 14 and CP 25 were expressed as ratios to the changes occurring in CP 17, very precise assay endpoints (λ=0.06−0.08) were obtained.