IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF A SALMONELLA-TYPHIMURIUM GENE INVOLVED IN TRIGGERING THE INTERNALIZATION OF SALMONELLAE INTO CULTURED EPITHELIAL-CELLS
IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF A SALMONELLA-TYPHIMURIUM GENE INVOLVED IN TRIGGERING THE INTERNALIZATION OF SALMONELLAE INTO CULTURED EPITHELIAL-CELLS
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DOI：
10.1073/pnas.89.13.5976
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发表时间：
1992-07-01
影响因子：
11.1
通讯作者：
GALAN, JE
中科院分区：
文献类型：
--
作者：
GINOCCHIO, C;PACE, J;GALAN, JE
肠上皮细胞的穿透是沙门氏菌感染发病机制中的重要步骤。我们的特点是一个基因，invE，这是必要的沙门氏菌入侵培养的上皮细胞。预测的InvE的氨基酸序列与耶尔森氏菌外膜蛋白YopN（LcrE）具有显著的同源性。携带invE突变的沙门氏菌菌株无法穿透Henle-407人肠细胞和Madin-Darby犬肾细胞，尽管它们完全能够附着在相同的细胞上。与野生型鼠伤寒沙门氏菌不同，invE突变体未能改变细胞内游离钙水平或聚合肌动蛋白在培养的上皮细胞中的分布;它们也没有改变极化Madin-Darby犬肾细胞微绒毛的正常结构。野生型S.鼠伤寒沙门氏菌能够在培养的上皮细胞的同时感染中拯救invE突变体的侵袭表型，尽管它不能拯救大肠杆菌菌株RDEC-1。我们假设invE突变体在触发导致细菌内化的细胞内事件方面存在缺陷。
Penetration of intestinal epithelial cells is an important step in the pathogenesis of Salmonella infections. We have characterized a gene, invE, that is necessary for Salmonella invasion of cultured epithelial cells. The predicted amino acid sequence of InvE showed significant homology to the Yersinia outer membrane protein YopN (LcrE). Strains of Salmonella carrying mutations in invE were unable to penetrate Henle-407 human intestinal cells and Madin-Darby canine kidney cells, although they were fully capable of attaching to the same cells. Unlike wild-type Salmonella typhimurium, invE mutants failed to change the intracellular free calcium levels or the distribution of polymerized actin in cultured epithelial cells; neither did they alter the normal architecture of the microvilli of polarized Madin-Darby canine kidney cells. Wild-type S. typhimurium was able to rescue the invasive phenotype of the invE mutants in simultaneous infections of cultured epithelial cells although it did not rescue the Escherichia coli strain RDEC-1. We hypothesize that invE mutants are deficient in triggering the intracellular events that lead to bacterial internalization.