Targeting pancreatic cancer with magneto-fluorescent theranostic gold nanoshells
Targeting pancreatic cancer with magneto-fluorescent theranostic gold nanoshells
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DOI：
10.2217/nnm.13.84
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发表时间：
2014-06-01
期刊：
影响因子：
5.5
通讯作者：
Joshi, Amit
中科院分区：
文献类型：
--
作者：
Chen, Wenxue;Ayala-Orozco, Ciceron;Joshi, Amit
目的：报道一种靶向中性粒细胞明胶酶相关脂蛋白(NGAL)的磁性荧光热感纳米复合体，用于胰腺癌的成像和治疗。材料与方法：将波长为810 nm的金纳米壳层包裹在掺杂氧化铁和近红外染料吲哚青绿的二氧化硅外延层中，得到热敏性金纳米壳(TGNS)，并与抗NGAL抗体偶联于ASPC-1裸鼠移植瘤中。结果：抗NGAL偶联的TGNS在体外和体内针对胰腺癌细胞提供了近红外荧光和T2加权磁共振成像的对比度，与使用长循环但非靶向的聚乙二醇化纳米粒获得的肿瘤对比度更高。这些纳米复合体还可以通过体外近红外光热疗法使高度特异的癌细胞死亡。结论：包埋近红外和磁共振对比剂的TGNS可特异性靶向表达早期疾病标志物NGAL的胰腺癌细胞，实现分子靶向成像和光热治疗。
Aim: We report a magneto-fluorescent theranostic nanocomplex targeted to neutrophil gelatinase-associated lipocalin (NGAL) for imaging and therapy of pancreatic cancer. Materials & methods: Gold nanoshells resonant at 810 nm were encapsulated in silica epilayers doped with iron oxide and the near-infrared (NIR) dye indocyanine green, resulting in theranostic gold nanoshells (TGNS), which were subsequently conjugated with antibodies targeting NGAL in AsPC-1-derived xenografts in nude mice. Results: Anti-NGAL-conjugated TGNS specifically targeted pancreatic cancer cells in vitro and in vivo providing contrast for both NIR fluorescence and T2-weighted MRI with higher tumor contrast than can be obtained using long-circulating, but nontargeted, PEGylated nanoparticles. The nanocomplexes also enabled highly specific cancer cell death via NIR photothermal therapy in vitro. Conclusion: TGNS with embedded NIR and magnetic resonance contrasts can be specifically targeted to pancreatic cancer cells with expression of early disease marker NGAL, and enable molecularly targeted imaging and photothermal therapy.