The actions of secretagogues on oxygen uptake by isolated mammalian parietal cells.
The actions of secretagogues on oxygen uptake by isolated mammalian parietal cells.
复制标题
促分泌素对分离的哺乳动物壁细胞吸氧的作用。
DOI：
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发表时间：
1978
影响因子：
15.9
通讯作者：
A. Soll
中科院分区：
文献类型：
--
作者：
A. Soll
研究了组胺、氨甲酰胆碱和胃泌素对犬基底粘膜细胞吸氧的影响。将分离的粘膜依次暴露于胶原酶和EDTA中制备有活力的粘膜细胞。用极谱法测定的耗氧量，作为粘膜细胞对促分泌剂生理反应的指标。异丁基甲基黄嘌呤（IMX）、氨甲酰胆碱、组胺和gastrín各自独立地刺激未分离的粘膜细胞的氧摄取。当IMX存在时，对组胺的反应大大增强。在用贝克曼洗脱器转子获得的不同壁细胞含量的分数中，基础和受激摄氧量与分数的壁细胞含量相关。组胺、胃泌素、氨甲酰胆碱和IMX对氧摄取的反应增加百分比在壁细胞含量为50%至85%的富集组分和未富集的起始组分中相似。具有IMX背景的组胺和氨甲酰胆碱的标准化剂量-反应关系在这两个组分中也相似。这些反应的特异性是通过使用H(2)-组胺受体拮抗剂甲胺和抗胆碱能剂阿托品来测试的。在使用的剂量下，甲氨酰胺（0.1 mM）和阿托品（10 muM）均未抑制基础摄氧量。在IMX背景下研究，组胺被甲氨酰胺抑制，而阿托品不抑制；氨甲酰胆碱被阿托品抑制，而甲氨酰胺不抑制。甲氨酰胺和阿托品均未抑制胃泌素刺激的摄氧量。这些数据表明，在这个体外系统中，由于暴露于胃分泌剂而产生的摄氧量增加主要是由壁细胞引起的，组胺、胃泌素和氨甲酰胆碱各自独立地刺激壁细胞的摄氧量。阿托品和甲氨酰胺在体外系统中显示的特异性表明，壁细胞对每一种分泌物都有特异性受体。
The action of histamine, carbamylcholine, and gastrin on oxygen uptake by cells isolated from canine fundic mucosa was studied in vitro. Viable mucosal cells were prepared by exposure of separated mucosa sequentially to collagenase and EDTA. Oxygen consumption, determined by polarography, was chosen as an index of physiological response of mucosal cells to secretagogues. Isobutyl methyl xanthine (IMX), carbamylcholine, histamine, and gastrín each independently stimulated oxygen uptake by the unfractionated mucosal cells. The response to histamine was greatly enhanced when IMX was present. In fractions of varying parietal cell content obtained with the Beckman elutriator rotor, basal and stimulated oxygen uptake correlated with the parietal cell content of the fractions. The percentage increases in oxygen uptake in response to histamine, gastrin, carbamylcholine, and IMX were similar in enriched fractions with from 50 to 85% parietal cells and in unenriched starting fractions. The normalized dose-response relations for histamine with an IMX background and for carbamylcholine were also similar in these two fractions.The specificity of these responses was tested by use of an H(2)-histamine receptor antagonist, metiamide, and an anticholinergic agent, atropine. At the doses used, neither metiamide (0.1 mM) nor atropine (10 muM) inhibited basal oxygen uptake. Histamine, studied with an IMX background, was inhibited by metiamide but not by atropine, while carbamylcholine was inhibited by atropine but not by metiamide. Neither metiamide nor atropine inhibited gastrin-stimulated oxygen uptake. These data indicate that in this in vitro system parietal cells account for most of the increase in oxygen uptake produced by exposure to gastric secretagogues and that histamine, gastrin, and carbamylcholine each independently stimulate oxygen uptake by the parietal cell. The specificity displayed by atropine and metiamide in this in vitro system suggests that the parietal cell has specific receptors for each of these secretagogues.