Effect of calcitonin gene-related peptide on the cyclic AMP level of isolated mouse diaphragm.
Effect of calcitonin gene-related peptide on the cyclic AMP level of isolated mouse diaphragm.
复制标题
降钙素基因相关肽对离体小鼠膈肌环AMP水平的影响。
DOI：
10.1254/jjp.42.345
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
H. Yoshida
中科院分区：
文献类型：
--
作者：
K. Takami;K. Hashimoto;S. Uchida;M. Tohyama;H. Yoshida
本文观察了降钙素基因相关肽（CGRP）对小鼠膈肌环核苷酸水平的影响。CGRP在浓度高达1 μ M时引起环AMP水平的剂量依赖性增加，但对环GMP水平没有影响。在1 μ M时，CGRP使环AMP水平增加约2.7倍。此外，在磷酸二酯酶抑制剂（Ro 20-1724）的存在下，CGRP仍然引起环AMP水平的更大剂量依赖性增加。即使在β-肾上腺素能拮抗剂普萘洛尔的存在下，CGRP刺激增加环AMP水平。此外，在小鼠膈肌中观察到CGRP的特异性结合。所有这些结果表明，CGRP通过CGRP受体而不是β-肾上腺素能受体增加细胞内cAMP水平。
The effect of calcitonin gene-related peptide (CGRP) on the cyclic nucleotide level in isolated mouse diaphragm was investigated. CGRP at concentrations of up to 1 microM caused dose-dependent increases in cyclic AMP levels but had no effect on cyclic GMP levels. At 1 microM, CGRP increased cyclic AMP levels by about 2.7-fold. Moreover, in the presence of phosphodiesterase inhibitor (Ro 20-1724), CGRP still caused even greater dose-dependent increases in cyclic AMP levels. Even in the presence of propranolol, a beta-adrenergic antagonist, CGRP stimulated increased cyclic AMP levels. In addition, specific binding of CGRP was observed in mouse diaphragm. All these results suggest that CGRP increases intracellular cyclic AMP levels via a CGRP receptor but not the beta-adrenergic receptor.