Sensitive and specific detection of pine nut (Pinus spp.) by real-time PCR in complex food products
Sensitive and specific detection of pine nut (Pinus spp.) by real-time PCR in complex food products
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DOI：
10.1016/j.foodchem.2015.08.114
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发表时间：
2016-03-01
期刊：
影响因子：
8.8
通讯作者：
Arlorio, Marco
中科院分区：
文献类型：
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作者：
Garino, Cristiano;De Paolis, Angelo;Arlorio, Marco
松子是一种已知的食物过敏原来源，已报道了几例食用后的不良免疫反应。为了保护过敏的消费者，必须开发出明确检测复杂基质中松子存在的方法。建立了一种基于taqman的实时PCR检测松属植物的方法。自制香蒜酱加入已知浓度的松子粉作为示范食品。此外，DNA是从宣称或不宣称松子存在的商业食品中纯化出来的。该方法对松属植物具有很高的特异性和效率。其内在LOD为1 pg DNA，而模型食品的实际LOD为0.1 ppm松子粉，这是通过实时PCR检测食物过敏原的最低记录。最后，证实了商业食品中存在/不存在松子。(C) 2015 Elsevier Ltd.版权所有。
Pine nuts are a known source of food allergens and several cases of adverse immunological reaction after ingestion have been reported. To protect allergic consumers, methods to unequivocally detect the presence of pine nuts in complex matrices must be developed.A Taqman-based real time PCR method for the detection of Pinus spp. was set up. A homemade pesto spiked at known concentration of pine nut powder was used as model food. Moreover, DNA was purified from commercial foods declaring or not the presence of pine nuts.The method displayed a very high efficiency and specificity for the genus Pinus. The intrinsic LOD was 1 pg of DNA, while the practical LOD evaluated on model foods was 0.1 ppm of pine nuts powder, the lowest ever registered for the detection of food allergens via real-time PCR. Finally, the declared presence/absence of pine nut in commercial foods was confirmed. (C) 2015 Elsevier Ltd. All rights reserved.