Tebuconazole induced oxidative stress related hepatotoxicity in adult and larval zebrafish (Danio rerio)
Tebuconazole induced oxidative stress related hepatotoxicity in adult and larval zebrafish (Danio rerio)
复制标题
戊唑醇诱导成年斑马鱼和幼体斑马鱼（斑马鱼）氧化应激相关的肝毒性
DOI：
10.1016/j.chemosphere.2019.125129
复制
发表时间：
2020-02-01
期刊：
影响因子：
8.8
通讯作者：
Zhu, Guonian
中科院分区：
文献类型：
--
作者：
Li, Shuying;Jiang, Yao;Zhu, Guonian
戊唑醇是一种广泛使用的杀菌剂，在环境介质中经常检测到高浓度。探讨戊唑醇对脊椎动物和人体的潜在毒性。以斑马鱼为动物模型，研究了低浓度戊唑醇对成年斑马鱼肝脏的毒性作用。我们进一步集中在tebuconazole诱导的毒性和其可能的机制，在幼斑马鱼肝毒性试验。通过测定超氧化物歧化酶（T-SOD）、过氧化氢酶（CAT）、过氧化物酶（POD）、谷胱甘肽S-转移酶（GST）活性以及天门冬氨酸氨基转移酶（AST）/丙氨酸氨基转移酶（ALT）比值的变化来评价氧化应激对成鱼的诱导作用。在暴露和净化阶段，雄性和雌性鱼肝脏中的酶活性均显著增加。受精后72 ~ 120 h暴露于最大非致死浓度戊唑醇（MNLC）对斑马鱼幼鱼肝脏大小和卵黄保留率有影响。在Tg（Apo 14：GFP）斑马鱼幼虫中观察到荧光强度降低，表明戊唑醇处理后肝脏退化。组织学检查证实了暴露斑马鱼肝脏组织结构的改变。MNLC组和LC 10组的活性氧水平分别增加了1.28倍和1.65倍。吖啶橙子染色显示肝区有凋亡细胞。这些结果表明，戊唑醇暴露对鱼类和脊椎动物的生态风险产生了影响。总之，本研究表明，需要进一步的研究，以更好地了解戊唑醇诱导的毒性机制，与氧化应激。(C)2019爱思唯尔有限公司版权所有。
Tebuconazole is widely used as fungicide and has frequently been detected at elevated concentrations in environmental media. To characterize the potential toxicity of tebuconazole on vertebrate and humans. Using zebrafish as a vertebrate model, the toxic effects in liver that produced by low-toxic concentrations of tebuconazole were assessed in adult zebrafish. We further focused on tebuconazole-induced toxicity and its possible mechanism in larval zebrafish using a hepatotoxicity assay. The induction of oxidative stress in adult fish was evaluated by superoxide dismutase (T-SOD), catalase (CAT), peroxidase (POD), glutathione S-transferase (GST) activity, and the increased aspartate aminotransferase (AST)/alanine aminotransferase (ALT) ratio. Significantly increased enzyme activity was observed in the liver of male and female fish at both exposure and depuration stage. Exposure to maximum non-lethal (MNLC) concentration of tebuconazole from 72 to 120 h post-fertilization (hpf) affected the liver size and yolk retention in larval zebrafish. Decreased fluorescence intensity was observed in larval Tg(Apo14:GFP) zebrafish, indicating liver degeneration after tebuconazole treated. Histopathological examination confirmed the alterations in liver histoarchitecture in exposed zebrafish. Significant 1.28-fold and 1.65-fold increases in reactive oxygen species levels were observed in juveniles exposed to MNLC and lethal concentration 10 (LC10) group, respectively. The acridine orange staining assay showed that apoptotic cells occurred in the liver regions. These results indicated that tebuconazole exposure resulted in impacts on the ecological risk in fish and vertebrate. Overall, the present study suggested further research in needed to better understand the tebuconazole-induced toxicity mechanism that associated with oxidative stress. (C) 2019 Elsevier Ltd. All rights reserved.