Genetic and molecular characterization of H9N2 and H5 avian influenza viruses from live poultry markets in Zhejiang Province, eastern China.
Genetic and molecular characterization of H9N2 and H5 avian influenza viruses from live poultry markets in Zhejiang Province, eastern China.
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中国东部浙江省活禽市场 H9N2 和 H5 禽流感病毒的遗传和分子特征
DOI：
10.1038/srep17508
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发表时间：
2015-12-02
影响因子：
4.6
通讯作者：
Wu N
中科院分区：
文献类型：
--
作者：
Wu H;Peng X;Peng X;Cheng L;Lu X;Jin C;Xie T;Yao H;Wu N
由于陆生和水生家禽的密度以及禽流感病毒感染的频率，活禽市场是重组禽流感病毒的主要来源。H9 N2病毒在整个亚洲的陆地家禽中流行，并已从世界各地的家禽疫情中分离出来。它们感染鸟类和哺乳动物物种，可能是新出现的人类病原体的重要遗传物质供体。2013-2014年对浙江省的LPM进行了AIV调查。从3，328份样本中分离出374株AIV。进行全基因组测序和系统发育分析。我们鉴定了一种新的H9 N2病毒基因型，其经历了与来自Qa/HK/G1/97样、Ck/BJ/1/94样和Dk/HK/Y 439/97样病毒的基因片段的重配。系统发育分析表明，H9 N2病毒经历了与其他AIV亚型的重组。浙江省H5亚型病毒存在2个不同进化枝（2.3.2和2.3.4.6）的混合流行。鉴于在鹅和鸭中检测到重组的H5禽流感病毒，可能是表面上健康的鸟类促成了新出现的H5禽流感病毒。中国东部的家禽需要继续监测。
Live poultry markets (LPMs) are a key source of reassorted avian influenza viruses (AIVs) because of the density of terrestrial and aquatic poultry and the frequency of AIV infection. H9N2 viruses are prevalent in terrestrial poultry throughout Asia and have been isolated from poultry outbreaks worldwide. They infect both avian and mammalian species and may be significant donors of genetic material to emerging human pathogens. LPMs in Zhejiang Province were surveyed from 2013–2014 for AIVs. Three hundred seventy-four (374) AIV strains were isolated from 3,328 samples. Whole–genome sequencing and phylogenetic analyses were performed. We identified a novel H9N2 virus genotype that had undergone reassortment with gene segments from Qa/HK/G1/97–like, Ck/BJ/1/94–like and Dk/HK/Y439/97–like viruses. Phylogenetic analyses suggested the H9N2 viruses had undergone reassortments with other AIV subtypes. The results also suggested that two different clades (2.3.2 and 2.3.4.6) of H5 viruses were co–circulating in Zhejiang Province. Given that reassorted H5 AIVs were detected in geese and ducks, it is possible that apparently healthy birds contribute to emerging H5 AIVs. Continued surveillance is required in poultry in eastern China.