Depletion-mediated red blood cell aggregation in polymer solutions
Depletion-mediated red blood cell aggregation in polymer solutions
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DOI：
10.1016/s0006-3495(02)75259-4
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发表时间：
2002-11-01
影响因子：
3.4
通讯作者：
Meiselman, HJ
中科院分区：
文献类型：
--
作者：
Neu, B;Meiselman, HJ
聚合物诱导的红细胞（RBC）聚集是目前的基础科学和临床的兴趣，并已提出了这种现象的消耗介导的模型，但到目前为止，这种模型的分析方法是缺乏的。因此，描述了一种用于计算聚合物溶液中RBC之间的相互作用能的方法。该模型结合了由于RBC表面电荷与渗透吸引力，由于聚合物耗尽附近的RBC表面的静电排斥。对于40至500 kDa的葡聚糖和18至35 kDa的聚（乙二醇），考虑了聚合物浓度和聚合物理化性质对耗尽层厚度和聚合物渗透到RBC糖萼中的影响。计算结果与文献数据细胞-细胞亲和力和红细胞聚集-聚合物浓度关系非常吻合。因此，这些研究结果提供了强有力的支持，耗尽相互作用的基础上聚合物诱导的红细胞聚集，并建议这种方法的有用性，探索大分子和红细胞糖萼之间的相互作用。
Polymer-induced red blood cell (RBC) aggregation is of current basic science and clinical interest, and a depletion-mediated model for this phenomenon has been suggested; to date, however, analytical approaches to this model are lacking. An approach is thus described for calculating the interaction energy between RBC in polymer solutions. The model combines electrostatic repulsion due to RBC surface charge with osmotic attractive forces due to polymer depletion near the RBC surface. The effects of polymer concentration and polymer physicochemical properties on depletion layer thickness and on polymer penetration into the RBC glycocalyx are considered for 40 to 500 kDa dextran and for 18 to 35 kDa poly (ethylene glycol). The calculated results are in excellent agreement with literature data for cell-cell affinities and with RBC aggregation-polymer concentration relations. These findings thus lend strong support to depletion interactions as the basis for polymer-induced RBC aggregation and suggest the usefulness of this approach for exploring interactions between macromolecules and the RBC glycocalyx.