Identification of Key Drug Targets and Molecular Mechanisms of Curcumae Rhizoma Acting on HBV-Related HCC: Weighted Correlation Network and Network Pharmacological Analyses.
Identification of Key Drug Targets and Molecular Mechanisms of Curcumae Rhizoma Acting on HBV-Related HCC: Weighted Correlation Network and Network Pharmacological Analyses.
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姜黄作用于HBV相关HCC的关键药物靶点和分子机制的鉴定：加权相关网络和网络药理学分析
DOI：
10.1155/2022/5399766
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Zhang G
中科院分区：
文献类型：
--
作者：
Zhao M;Fu Y;Liu L;Hou Y;Shi M;Zhou H;Zhang G
背景：乙肝病毒相关性肝细胞癌预后差，病死率高。姜黄是一种经典的中草药，常用于治疗肿瘤。方法从中药数据库和分析平台(TCMSP)数据库中提取郁金有效成分，利用TCMSP数据库和瑞士目标预测数据库预测潜在靶点。通过交叉预测靶点、DEG和WGCNA中重要模块中的基因来筛选关键药物靶点。此外，利用关键药物靶点构建了“草药活性成分-靶点-疾病”相互作用网络，并进行了浓缩分析和蛋白质-蛋白质相互作用(PPI)分析。基于PPI分析的HUB目标通过KMplotter数据库进行评估。结果用30%的OB ≥ 和0 18%的DL ≥ 分离得到3种有效成分，分别为长春花素、文氏剂和双去甲氧基姜黄素。关键药物靶点主要集中在细胞周期检查点、DNA完整性检查点和多肽-丝氨酸修饰。此外，姜黄对乙肝相关性肝癌的治疗主要涉及P53信号通路和花生四烯酸代谢。最后，PPI分析确定ESR1和Ptgs2为HUB靶标。研究发现，ESR1与肝癌合并肝炎患者的生存期相关。结论基于WGCNA和网络药理学分析，姜黄可能通过多靶点、多途径发挥作用。
Background Hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) has poor prognosis and high mortality rate. Curcumae Rhizoma, a classic Chinese medicinal herb, is often used to treat tumors. Methods Active ingredients of Curcumae Rhizoma were extracted from the Traditional Chinese Medicine Database and Analysis Platform (TCMSP) database, and potential targets were predicted by the TCMSP database and Swiss Target Prediction database. The key drug targets were filtered by intersecting predicted targets, DEGs, and genes in important modules from WGCNA. Besides, the key drug targets were used to construct a network of “herb-active ingredient-target-disease” interactions and subjected to enrichment analysis and protein-protein interaction (PPI) analysis. The hub targets based on PPI analysis was evaluated by the KMplotter database. Results Three active ingredients of Curcumae Rhizoma were collected with OB ≥ 30% and DL ≥ 0.18, including hederagenin, wenjine, and bisdemethoxycurcumin. The key drug targets were mainly enriched in cell cycle checkpoint, DNA integrity checkpoint, and peptidyl-serine modification. Besides, Curcumae Rhizoma treatment of HBV-related HCC mainly involved the p53 signaling pathway and arachidonic acid metabolism. Finally, ESR1 and PTGS2 were identified as hub targets from PPI analysis. ESR1 was found to be correlated with survival in liver cancer patients with hepatitis. Conclusion Based on WGCNA and network pharmacological analysis, our results illustrated that Curcumae Rhizoma might work through regulating multitargets and multipathways in HBV-related HCC.
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影响因子：
5.6
作者：
Hsia TC;Peng SF;Chueh FS;Lu KW;Yang JL;Huang AC;Hsu FT;Wu RS
通讯作者：
Wu RS
影响因子：
5.8
作者：
Gu, Zuguang;Eils, Roland;Schlesner, Matthias
通讯作者：
Schlesner, Matthias
WGCNA：用于加权相关网络分析的 R 包。
影响因子：
3
作者：
Langfelder P;Horvath S
通讯作者：
Horvath S
肝癌的发生需要通过 CD44 增强的生长因子信号传导抑制 p53
影响因子：
50.3
作者：
Dhar D;Antonucci L;Nakagawa H;Kim JY;Glitzner E;Caruso S;Shalapour S;Yang L;Valasek MA;Lee S;Minnich K;Seki E;Tuckermann J;Sibilia M;Zucman-Rossi J;Karin M
通讯作者：
Karin M
影响因子：
4.7
作者：
Li, Wenli;Wang, Huimei;Liu, Jun
通讯作者：
Liu, Jun