Variability in the analysis of 25-hydroxyvitamin D by liquid chromatography-tandem mass spectrometry: The devil is in the detail
Variability in the analysis of 25-hydroxyvitamin D by liquid chromatography-tandem mass spectrometry: The devil is in the detail
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DOI：
10.1016/j.cca.2012.04.003
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发表时间：
2012-08-16
影响因子：
5
通讯作者：
Moniz, Cajetan F.
中科院分区：
文献类型：
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作者：
Couchman, Lewis;Benton, Christopher M.;Moniz, Cajetan F.
背景：液相色谱串联质谱仪(LC-MS/MS)越来越多地应用于临床实验室中25-羟基维生素D(25OHD)的分析，但测定方法并不简单。重要的是。LC-MS/MS不是一种单一的技术：样品制备、层析和电离/裂解过程中的变量都应该被考虑。方法：我们分析了国际维生素D室内质量评估计划(DEQAS)组织的一项调查结果，以确定这些变量对两个DEQAS分布结果的影响。结果：65个实验室收回了问卷。346(57%)个人的结果来自使用电喷雾电离(ESI)的实验室，259个(43%)来自使用大气压化学电离(APCI)的实验室。两组的平均结果比差异无统计学意义(P=0.5828)，但差异有统计学意义(P
Background: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is increasingly used in clinical laboratories for the analysis of 25-hydroxyvitamin D (25OHD), but measurement is not straightforward. Importantly. LC-MS/MS is not a single technique: variables in sample preparation, chromatography and ionisation/fragmentation should each be considered.Methods: We analysed results from a survey organised by the international Vitamin D External Quality Assessment Scheme (DEQAS), to determine the influence of such variables on the results for two DEQAS distributions.Results: 65 laboratories returned questionnaires. 346 (57%) individual results were from laboratories using electrospray ionisation (ESI), and 259 (43%) from laboratories using atmospheric pressure chemical ionisation (APCI). Although the mean ratio of results was not significantly different between ESI and APCI (P = 0.5828), there was greater variation (P