Cytotoxicity of red fluorescent protein DsRed is associated with the suppression of Bcl-xL translation
Cytotoxicity of red fluorescent protein DsRed is associated with the suppression of Bcl-xL translation
复制标题
红色荧光蛋白 DsRed 的细胞毒性与 Bcl-xL 翻译抑制有关
DOI：
10.1016/j.febslet.2011.02.013
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发表时间：
2011-03-09
期刊：
影响因子：
3.5
通讯作者：
Xia, Bin
中科院分区：
文献类型：
--
作者：
Zhou, Jun;Lin, Jian;Xia, Bin
红色荧光蛋白（RFP）DsRed及其变体广泛应用于活细胞成像实验。然而，限制DsRed应用的一个主要因素是其细胞毒性。在这里，我们报告，DsRed及其变体DsRed-xL 2抑制B细胞淋巴瘤超大（Bcl-xL）在HeLa细胞中的表达通过翻译调节。过表达Bcl-xL可降低DsRed的细胞毒性。同时，Turbo RFP，一种来自四色Entacmaea quadricolor的突变RFP，不影响Bcl-xL的表达，这表明来自不同物种的RFP的细胞毒性机制可能不同。我们的研究结果揭示了DsRed细胞毒性的可能机制，为提高DsRed及其变体的应用提供了潜在的策略。(C)2011年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
Red fluorescent protein (RFP) DsRed and its variants are widely applied in live-cell imaging experiments. However, a major factor that restricts the application of DsRed is its cytotoxicity. Here, we report that DsRed and its variant DsRed-Express2 inhibit the expression of B-cell lymphoma-extra large (Bcl-xL) in HeLa cells by translational regulation. Over-expression of Bcl-xL can reduce the cytotoxicity of DsRed. Meanwhile, Turbo RFP, a mutant RFP from Entacmaea quadricolor, does not affect Bcl-xL expression, suggesting that cytotoxic mechanisms of RFP from different species may be varied. Our results reveal a possible mechanism for the cytotoxicity of DsRed, providing a potential strategy to improve the application of DsRed and its variants. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.