Heritable Custom Genomic Modifications in Caenorhabditis elegans via a CRISPR-Cas9 System
Heritable Custom Genomic Modifications in Caenorhabditis elegans via a CRISPR-Cas9 System
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DOI：
10.1534/genetics.113.156075
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发表时间：
2013-11-01
期刊：
影响因子：
3.3
通讯作者：
Colaiacovo, Monica P.
中科院分区：
文献类型：
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作者：
Tzur, Yonatan B.;Friedland, Ari E.;Colaiacovo, Monica P.
我们调整了CRISPR-Cas9系统，用于在秀丽隐杆线虫中通过同源重组对靶向双链断裂进行模板介导的修复，从而实现定制和高效的基因组编辑。该系统可用于在C.优雅的
We adapted the CRISPR-Cas9 system for template-mediated repair of targeted double-strand breaks via homologous recombination in Caenorhabditis elegans, enabling customized and efficient genome editing. This system can be used to create specific insertions, deletions, and base pair changes in the germline of C. elegans.