Assembly of DNA-functionalized nanoparticles in alcoholic solvents reveals opposite thermodynamic and kinetic trends for DNA hybridization.
Assembly of DNA-functionalized nanoparticles in alcoholic solvents reveals opposite thermodynamic and kinetic trends for DNA hybridization.
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DOI：
10.1021/ja101973g
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发表时间：
2010-04
影响因子：
15
通讯作者：
Brendan D. Smith;Juewen Liu
中科院分区：
文献类型：
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作者：
Brendan D. Smith;Juewen Liu
DNA 一直是生物技术和纳米技术中的关键分子。迄今为止，大多数涉及 DNA 的实验都是在水溶液中进行的，这可能与 DNA 杂交在有机溶剂中速度较慢且稳定性较差的观点有关。所有关于有机溶剂影响的研究都集中在热力学性质上，例如 DNA 熔解温度和非常长 DNA 的 B 到 A 形式的转变，而不是短合成 DNA 的杂交动力学。我们采用 DNA 功能化金纳米粒子 (AuNP) 作为模型系统，发现如果酒精含量低于约 30%，更多的酒精会导致 DNA 杂交更快，但熔化温度会降低。这一观察结果的普遍性通过使用荧光团和猝灭剂标记 DNA 的两个分子信标系统（在没有 AuNP 的情况下）进行了独立验证。使用 25% 乙醇时，杂交速率比使用水时快三到四倍。这一发现将 DNA 生物和纳米技术的应用扩展到具有改进性能的有机溶剂。
DNA has been a key molecule in biotechnology and nanotechnology. To date, the majority of the experiments involving DNA have been performed in aqueous solutions, which may be related to the perception that DNA hybridization is slower and less stable in organic solvents. All studies on the effect of organic solvents have focused on thermodynamic properties such as DNA melting temperature and the B-to-A form transition for very long DNAs, but not on the hybridization kinetics of short synthetic DNAs. We employed DNA-functionalized gold nanoparticles (AuNPs) as a model system and found that if the alcohol content is less than approximately 30%, more alcohol leads to a faster DNA hybridization, although with a decreased melting temperature. The generality of this observation was independently verified with two molecular beacon systems (in the absence of AuNPs) using fluorophore and quencher-labeled DNAs. With 25% ethanol, the hybridization rates are three to four times faster than in the case with water. This discovery will extend the application of DNA bio- and nanotechnology to organic solvents with improved performance.