Virus-Induced Gene Silencing in Lilies Using Cucumber Mosaic Virus Vectors.
Virus-Induced Gene Silencing in Lilies Using Cucumber Mosaic Virus Vectors.
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DOI：
10.1007/978-1-0716-0751-0_1
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发表时间：
2020
影响因子：
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通讯作者：
Keisuke Tasaki;M. Yamagishi;C. Masuta
中科院分区：
文献类型：
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作者：
Keisuke Tasaki;M. Yamagishi;C. Masuta
病毒诱导的基因沉默（VIGS）系统是快速分析百合等需要长时间生长的植物基因功能的有效方法。我们成功地建立了一个利用黄瓜花叶病毒（HL strain，CMV-HL）载体的VIGS系统。leichtliniii-phytoene desaturase基因（LlPDS），在接种后30天（dpi），在L. Leichtlinii，57 dpi时，花被片上出现白色区域，积累橙子类胡萝卜素。该载体可在鳞茎中传播，并可在次年萌发的芽中诱导沉默。CMV-HL载体可以通过将30-60 nt片段插入RNA 3基因组的克隆位点而容易地构建。在本章中，我们描述了如何在百合植物中使用CMV-HL载体系统。
Virus-induced gene silencing (VIGS) systems are effective for rapid analysis of gene functions in plants that require a long period of growth such asLilium. We successfully developed a VIGS system using the cucumber mosaic virus (HL strain, CMV-HL) vector to induce RNA silencing of theL. leichtliniiphytoene desaturase gene (LlPDS), where at 30 days postinoculation (dpi), photo-bleaching was observed in the upper leaves ofL. leichtlinii, and at 57 dpi, white regions appeared on flower tepals that accumulate orange carotenoids. This vector spreads in bulbs, and it could induce silencing on emerged shoots in the following year. The CMV-HL vector can be easily constructed by insertion of a 30–60 nt fragment into the cloning site of the RNA3 genome. In this chapter, we describe how to use the CMV-HL vector system in the context ofLiliumplants.