Effects of saliva collection using cotton swab on cortisol enzyme immunoassay
Effects of saliva collection using cotton swab on cortisol enzyme immunoassay
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DOI：
10.1007/s00421-009-1178-3
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发表时间：
2009-12-01
影响因子：
3
通讯作者：
Tochihara, Yutaka
中科院分区：
文献类型：
--
作者：
Kozaki, Tomoaki;Hashiguchi, Nobuko;Tochihara, Yutaka
棉签是最常用的唾液收集设备之一，但各种研究报告称，它们的使用会影响唾液皮质醇的检测结果。然而，这些研究通过比较浓度和/或散点图的平均值来估计这种影响。在本研究中，我们用Bland-Altman图估计了棉签对唾液皮质醇酶免疫分析(EIA)结果的影响。8名健康男性(年龄20-23岁)在不同的日期提供了4个唾液样本，总共产生了32个样本。唾液样本用塑料吸管直接收集在塑料管中，然后用吸管吸到棉签上(棉花唾液收集)，然后放入透明的无菌管中(被动唾液收集)。棉花与被动采集唾液的相关性较低。就个人而言，四名受试者表现出被动收集唾液和棉花收集之间的负相关。Bland-Altman图表明，棉签对EIA测定结果有一定的比例偏差。我们的发现表明，用棉签采集唾液有相当大的效果，而且影响的对象不同。Bland-Altman的情节进一步暗示了这种效应的可能原因。
Cotton swabs are among the most commonly used devices for collecting saliva, but various studies have reported that their use impacts the results of salivary cortisol assays. These studies, however, estimated this impact by comparing the average of the concentration and/or scatter plots. In the present study, we estimated the impact of cotton swabs on the results of salivary cortisol enzyme immunoassay (EIA) by Bland-Altman plot. Eight healthy males (aged 20-23 years) provided four saliva samples on different days to yield a total of 32 samples. Saliva samples were collected directly in plastic tubes using plastic straws and then pipetted onto cotton swabs (cotton saliva collection) and into clear sterile tubes (passive saliva collection). There was a lower correlation between cotton and passive saliva collection. Individually, four subjects showed a negative correlation between passive and cotton saliva collection. A Bland-Altman plot indicated that cotton swabs causes a proportional bias on the EIA assay result. Our findings indicate a considerable effect of using cotton swabs for saliva collection, and subject-specific variability in the impact. A Bland-Altman plot further suggests possible reasons for this effect.