In Vitro Evaluation of Anti-biofilm Agents Against Salmonella enterica.
In Vitro Evaluation of Anti-biofilm Agents Against Salmonella enterica.
复制标题
针对肠沙门氏菌的抗生物膜剂的体外评价。
DOI：
10.1007/978-1-0716-0791-6_12
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Gunn,JohnS
中科院分区：
文献类型：
--
作者：
Sandala,Jenna;Gunn,JohnS
肠道沙门氏菌能够建立强大的粘附社区，称为生物膜，允许生物和非生物表面的长期定殖。这些生物膜群落对从污染表面和感染宿主中成功根除细菌构成了重大挑战，因为进入生物膜表型赋予细菌群体对各种环境和治疗性损伤的耐受性，否则它将易受这些环境和治疗性损伤的影响。因此，确定特异性靶向沙门氏菌唇膜状态的抗菌策略对于预防和治疗生物膜介导的疾病非常重要。在这里，我们提供了详细的方法，在体外培养ofSalmonellabiofilms，可以很容易地扩大用于高通量筛选候选抗生物膜剂。这些测定还可用于进一步表征先导抗生物膜剂的抑制和/或破坏能力，以及鉴定证明增强的抗生物膜作用的组合治疗。此外，可以稍微修改测定（例如，最佳生长条件）以评价其它细菌属。
Salmonella entericais able to establish robust adherent communities called biofilms that allow for long-term colonization of both biotic and abiotic surfaces. These biofilm communities pose a significant challenge to successful eradication of the bacteria from contaminated surfaces and the infected host, as entry into the biofilm phenotype confers the bacterial population with tolerance to a variety of environmental and therapeutic insults to which it would otherwise be susceptible. The identification of antimicrobial strategies that specifically target theSalmonellabiofilm state is therefore of great importance in order to both prevent and treat biofilm-mediated disease. Here, we provide detailed methods for the in vitro cultivation ofSalmonellabiofilms that can easily be scaled up for use in high-throughput screening of candidate anti-biofilm agents. These assays may also be utilized to further characterize the inhibitory and/or disruptive capabilities of lead anti-biofilm agents, as well as to identify combination treatments that demonstrate enhanced anti-biofilm effects. Furthermore, the assays may be slightly modified (e.g., optimal growth conditions) to evaluate other bacterial genera.