Effective activation of resting mouse T lymphocytes by cross‐linking submitogenic concentrations of the T cell antigen receptor with either Lyt‐2 or L3T4
Effective activation of resting mouse T lymphocytes by cross‐linking submitogenic concentrations of the T cell antigen receptor with either Lyt‐2 or L3T4
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通过将 T 细胞抗原受体与 Lyt-2 或 L3T4 交联，有效激活静息小鼠 T 淋巴细胞
DOI：
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发表时间：
1987
影响因子：
5.4
通讯作者：
F. Emmrich
中科院分区：
文献类型：
--
作者：
K. Eichmann;J. Jönsson;I. Falk;F. Emmrich
我们研究了T细胞抗原受体抗体联合其他T细胞表面抗原抗体对小鼠静息T淋巴细胞的激活作用。固相非可溶性抗体KJ16133和F23.1都针对Vβ8家族的β链，在生长因子存在的情况下以剂量依赖的方式激活T细胞增殖。Lyt-2和L3T4抗体在任何浓度下均无激活作用。然而，亚促分裂浓度的KJ16-133和F23.1与多种浓度的抗Lyt-2和抗L3T4协同作用，可引起与高浓度抗T细胞受体抗体相似或更大的T细胞增殖。抗Lyt-1、LFA-1和H-2 I类抗原的抗体也有协同激活作用，但激活程度较低。这一点在限制性稀释实验中尤为明显，在该实验中，BALB/c T细胞对低剂量抗T细胞受体抗体和抗Lyt-2的校正增殖频率为1/4至1/7。BALB/c T细胞单独对高浓度抗T细胞受体抗体的反应率为1/14~1/126，与抗LFA-1或抗Lyt-1协同反应时T细胞增殖频率更低。协同激活可诱导功能性细胞毒细胞的产生。我们将这些数据解释为T细胞抗原受体与Lyt-2(CD8)或L3T4(CD4)的交联是静止T细胞的最佳激活信号。我们认为，在生理T细胞活化中，T细胞受体与CD8或CD4的交联性是由于它们同时与刺激细胞上的主要组织相容性复合体(MHC)I类(CD8)或MHC II类(CD4)分子结合而引起的。我们认为，在个体发育中的T细胞谱系选择过程中，也可能存在类似的交联要求，从而解释了MHC I类和II类限制性T细胞受体分别与CD8和CD4分子严格共表达的原因。
We studied the activation of small resting mouse T lymphocytes by antibodies to the T cell antigen receptor in combination with antibodies to other T cell surface antigens. Solid‐phase but not soluble antibodies KJ16‐133 and F23.1, both directed to β chains of the Vβ8 family, activate T cells to proliferate in the presence of growth factors, in a dose‐dependent fashion. Antibodies to Lyt‐2 and to L3T4 had no activating effect at any concentration. However, submitogenic concentrations of KJ16‐133 and of F23.1 synergized with a wide range of concentrations of anti‐Lyt‐2 and anti‐L3T4 to cause T cell proliferation similar or greater in magnitude to that caused by high concentrations of anti‐T cell receptor antibody. Synergistic activation was also observed with antibodies to Lyt‐1, LFA‐1 and H‐2 class I antigens but to a significantly lower degree. This was particularly clear in limiting dilution experiments in which the corrected frequencies of T cells proliferating in response to low amounts of anti‐T cell receptor antibody together with anti‐Lyt‐2 were 1/4 to 1/7 for BALB/c T cells. The frequencies of BALB/c T cells responding to high concentrations of anti‐T cell receptor antibody alone were between 1/14 and 1/126 and still lower frequencies of T cells proliferated in synergistic responses with anti‐LFA‐1 or anti‐Lyt‐1. Synergistic activation leads to the induction of functional cytotoxic cells. We interpret these data as suggestive that cross‐linking of the T cell antigen receptor with either Lyt‐2 (CD8) or L3T4 (CD4) represents an optimal activating signal for resting T cells. We think that, in physiological T cell activation, cross‐linking of the T cell receptor to CD8 or CD4 is induced by their simultaneous binding to major histocompatibility complex (MHC) class I (for CD8) or MHC class II (for CD4) molecules on stimulator cells. We consider the possibility that similar cross‐linking requirements may also exist during T cell repertoire selection in ontogeny, thus accounting for the strict coexpression of MHC class I and class II‐restricted T cell receptors with CD8 and CD4 molecules, respectively.
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L3T4 单克隆抗体可阻断 2 类主要组织相容性复合体抗原特异性 T 细胞的功能。
DOI：
--
发表时间：
1984
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Swain,SL;Dialynas,DP;Fitch,FW;English,M
通讯作者：
English,M
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DOI：
--
发表时间：
1985
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Schwab,R;Crow,MK;Russo,C;Weksler,ME
通讯作者：
Weksler,ME
DOI：
10.1073/pnas.79.14.4395
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
MEUER, SC;SCHLOSSMAN, SF;REINHERZ, EL
通讯作者：
REINHERZ, EL
OKT8 抗体抑制 OKT3 诱导的 OKT8 细胞中 IL 2 的产生和增殖。
DOI：
--
发表时间：
1983
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Welte,K;Platzer,E;Wang,CY;RinnooyKan,EA;Moore,MA;Mertelsmann,R
通讯作者：
Mertelsmann,R
人类 T 淋巴细胞亚群的激活：辅助和抑制/细胞毒性 T 细胞识别不同的组织相容性抗原并对其做出反应。
DOI：
--
发表时间：
1981
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Engleman,EG;Benike,CJ;Grumet,FC;Evans,RL
通讯作者：
Evans,RL