Outer hair cell-specific prestin-CreERT2 knockin mouse lines.
Outer hair cell-specific prestin-CreERT2 knockin mouse lines.
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作者：
Fang, Jie;Zhang, Wen-Cheng;Yamashita, Tetsuji;Gao, Jiangang;Zhu, Min-Sheng;Zuo, Jian
耳蜗的外毛细胞（OHC）对于听觉灵敏度和频率调谐至关重要。为了了解OHC的生理学和病理学，必须使用小鼠遗传工具来操纵OHC中的基因表达。在此，我们产生了2个普雷斯廷敲入小鼠系：1）普雷斯丁-CreERT 2系，其中内部核糖体进入位点（IRES）-CreERT 2-FRT-Neo-FRT盒插入到普雷斯廷基因座的终止密码子之后，和2）普雷斯丁-CreERT 2-NN系，随后去除FRT-Neo-FRT。我们通过将它们与报告细胞系CAG-eGFP和Ai 6杂交来表征两个细胞系的诱导型Cre活性。Cre活性诱导与他莫昔芬在不同的出生后年龄，只检测到OHC，类似的内源性普雷斯廷的表达模式。此外，prestin-CreERT 2 +/−（杂合子）和+/+（纯合子）以及prestin-CreERT 2-NN +/−小鼠表现出正常的听力。因此，这2个prestin-CreERT 2小鼠系是分析体内OHC中基因功能的有用工具。
Outer hair cells (OHCs) in the cochlea are crucial for the remarkable hearing sensitivity and frequency tuning. To understand OHC physiology and pathology, it is imperative to use mouse genetic tools to manipulate gene expression specifically in OHCs. Here, we generated 2 prestin knockin mouse lines: 1) the prestin-CreERT2 line, with an internal ribosome entry site (IRES)-CreERT2-FRT-Neo-FRT cassette inserted into the prestin locus after the stop codon, and 2) the prestin-CreERT2-NN line, with the FRT-Neo-FRT removed subsequently. We characterized the inducible Cre activity of both lines by crossing them with the reporter lines CAG-eGFP and Ai6. Cre activity was induced with tamoxifen at various postnatal ages and only detected in OHCs, resembling the endogenous prestin expression pattern. Moreover, prestin-CreERT2 +/− (heterozygotes) and +/+ (homozygotes) as well as prestin-CreERT2-NN +/− mice displayed normal hearing. These 2 prestin-CreERT2 mouse lines are therefore useful tools to analyze gene function in OHCs in vivo.
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影响因子：
2.8
作者：
Davis, Rickie R.;Kuo, Ming-Wen;Alagramam, Kumar N.
通讯作者：
Alagramam, Kumar N.
影响因子：
14.9
作者：
Indra, AK;Warot, X;Metzger, D
通讯作者：
Metzger, D
DOI：
10.1006/bbrc.1997.7124
发表时间：
1997-08-28
影响因子：
3.1
作者：
Feil, R;Wagner, J;Chambon, P
通讯作者：
Chambon, P
影响因子：
2.9
作者：
Tian, Yong;James, Sally;Beisel, Kirk W.
通讯作者：
Beisel, Kirk W.
影响因子：
25
作者：
Madisen L;Zwingman TA;Sunkin SM;Oh SW;Zariwala HA;Gu H;Ng LL;Palmiter RD;Hawrylycz MJ;Jones AR;Lein ES;Zeng H
通讯作者：
Zeng H