Effect of cell cycle stage, dose rate and repair of sublethal damage on radiation-induced apoptosis in F9 teratocarcinoma cells.
Effect of cell cycle stage, dose rate and repair of sublethal damage on radiation-induced apoptosis in F9 teratocarcinoma cells.
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DOI：
10.2307/3579240
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发表时间：
1995-10
影响因子：
3.4
通讯作者：
R. E. Langley;S. Quartuccio;P. Kennealey;C. Coleman;E. Bump
中科院分区：
文献类型：
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作者：
R. E. Langley;S. Quartuccio;P. Kennealey;C. Coleman;E. Bump
在电离辐射治疗后，至少有两种不同的细胞死亡模式。第一种是尽管新陈代谢存活，但未能进行持续的细胞分裂，我们将这种终点称为“经典的生殖细胞死亡”。第二个是导致细胞完整性丧失的过程。这第二类包括细胞坏死和细胞凋亡。我们实验室早期的研究表明，照射后的F9细胞的主要死亡机制是凋亡，没有迹象表明这些细胞是通过坏死而死亡的。因此，我们使用该细胞系的细胞来重新评估与细胞凋亡有关的基本放射生物学原理。经典的生殖细胞死亡通过对来自照射细胞的集落进行染色，并将少于50个细胞的集落计分为生殖性死亡，将超过50个细胞的集落计分为存活。未能产生任何一种集落(从平板上分离或解体)的细胞被评分为经历了凋亡。使用这些标准，我们发现，尽管总体存活率存在很大差异，但在细胞周期的不同阶段对细胞进行照射时，因辐射而死亡的F9细胞的比例没有变化。这表明在整个细胞周期中影响辐射敏感性的因素对细胞凋亡和经典的生殖细胞死亡具有同等的影响。分次剂量和单次剂量X射线照射后细胞存活率无明显差异，提示亚致死性损伤修复不是辐射诱导F9细胞凋亡的一个因素。剂量率在0.038~4.96Gymin变化对细胞凋亡无明显影响。
There are at least two different modes of cell death after treatment with ionizing radiation. The first is a failure to undergo sustained cell division despite metabolic survival, and we refer to this end point as "classical reproductive cell death." The second is a process that results in loss of cell integrity. This second category includes cellular necrosis as well as apoptosis. Earlier studies in our laboratory showed that the predominant mechanism of cell death for irradiated F9 cells is apoptosis, and there is no indication that these cells die by necrosis. We have therefore used cells of this cell line to reassess basic radiobiological principles with respect to apoptosis. Classical reproductive cell death was determined by staining colonies derived from irradiated cells and scoring colonies of less than 50 cells as reproductively dead and colonies of more than 50 cells as survivors. Cells that failed to produce either type of colony (detached from the plate or disintegrated) were scored as having undergone apoptosis. Using these criteria we found that the fraction of the radiation-killed F9 cells that died by apoptosis did not vary when cells were irradiated at different stages of the cell cycle despite large variations in overall survival. This suggests that the factors that influence radiation sensitivity throughout the cell cycle have an equal impact on apoptosis and classical reproductive cell death. There was no difference in cell survival between split doses and single doses of X rays, suggesting that sublethal damage repair is not a factor in radiation-induced apoptosis of F9 cells. Apoptosis was not affected by changes in dose rate in the range of 0.038-4.96 Gy/min.