Single and double metallothionein knockout in the nematode C-elegans reveals cadmium dependent and independent toxic effects on life history traits
Single and double metallothionein knockout in the nematode C-elegans reveals cadmium dependent and independent toxic effects on life history traits
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DOI：
10.1016/j.envpol.2006.06.003
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发表时间：
2007-01-01
影响因子：
8.9
通讯作者：
Sturzenbaum, Stephen R.
中科院分区：
文献类型：
--
作者：
Hughes, Sam;Sturzenbaum, Stephen R.
线虫秀丽隐杆线虫的基因组含有两个金属硫蛋白基因，两者都参与金属稳态和/或解毒。单金属硫蛋白敲除突变体已经被创建，现在，首次分离出双突变体。在存在或不存在镉的情况下的生活史研究表明，所有金属硫蛋白突变体都是可行的。尽管镉不影响寿命，但在野生型动物中观察到总巢体大小和体积生长的剂量依赖性减少，这种减少在单敲除中放大，在双敲除中进一步加剧。然而，金属硫蛋白缺失引起了两种与镉暴露无关的效应，即所有敲除菌株都表现出总巢大小减小，并且两个金属硫蛋白基因座的缺失导致体积生长显着减少。总之，金属硫蛋白无疑是镉解毒的重要参与者，但显然也是镉非依赖性途径的重要因素。 (c) 2006 Elsevier Ltd. 保留所有权利。
The genome of the nematode Caenorhabditis elegans contains two metallothionein genes, both involved in metal homeostasis and/or detoxification. Single metallothionein knockout mutants have been created and now, for the first time, a double mutant has been isolated. Life history studies in the presence or absence of cadmium showed that all metallothionein mutants are viable. Although cadmium did not influence longevity, a dose dependent reduction in total brood size and volumetric growth was observed in wild type animals, which was magnified in single knockouts and further exacerbated in the double knockout. However, the metallothionein deletion caused two effects that are independent of cadmium exposure, namely all knockout strains displayed a reduced total brood size and the deletion of both metallothionein loci caused a significant reduction in volumetric growth. In summary, metallothionein is undoubtedly an important player in cadmium detoxification, but evidently also an important factor in cadmium independent pathways. (c) 2006 Elsevier Ltd. All rights reserved.