The Use of Single-Cell RNA-Sequencing and Spatial Transcriptomics in Understanding the Pathogenesis and Treatment of Skin Diseases.
The Use of Single-Cell RNA-Sequencing and Spatial Transcriptomics in Understanding the Pathogenesis and Treatment of Skin Diseases.
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DOI：
10.1016/j.xjidi.2023.100198
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发表时间：
2023-07
期刊：
影响因子：
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通讯作者：
Ji, Andrew L
中科院分区：
文献类型：
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作者：
Houser, Aubrey E;Kazmi, Abiha;Nair, Arjun K;Ji, Andrew L
近年来，多组学分析工具的发展迅速扩大，沿着它们在各种情况下（包括皮肤病）分析皮肤组织。在这些工具中，单细胞RNA测序（scRNA-seq）和空间转录组学（ST）已成为广泛采用的强大检测方法，用于阐明皮肤疾病中的关键细胞组分及其空间排列。在本文中，我们回顾了最近从scRNA-seq和ST的使用中获得的生物学见解，以及将两者结合用于分析皮肤疾病的优势，包括异常伤口愈合，炎症性皮肤病和癌症。我们讨论了scRNA-seq和ST在改善皮肤病治疗方面的作用，并朝着实现皮肤病学精准医学的目标迈进，从而使患者能够最佳地匹配最大化治疗反应的治疗。
The development of multiomic profiling tools has rapidly expanded in recent years, along with their use in profiling skin tissues in various contexts, including dermatologic diseases. Among these tools, single-cell RNA-sequencing (scRNA-seq) and spatial transcriptomics (ST) have emerged as widely adopted and powerful assays for elucidating key cellular components and their spatial arrangement within skin disease. In this paper, we review the recent biological insights gained from the use of scRNA-seq and ST and the advantages of combining both for profiling skin diseases, including aberrant wound healing, inflammatory skin diseases, and cancer. We discuss the role of scRNA-seq and ST in improving skin disease treatments and moving toward the goal of achieving precision medicine in dermatology, whereby patients can be optimally matched to treatments that maximize therapeutic response.