Combining multispectral polarized light imaging and confocal microscopy for localization of nonmelanoma skin cancer.
Combining multispectral polarized light imaging and confocal microscopy for localization of nonmelanoma skin cancer.
复制标题
结合多光谱偏振光成像和共焦显微镜来定位非黑色素瘤皮肤癌。
DOI：
10.1117/1.1854173
复制
发表时间：
2005
影响因子：
3.5
通讯作者：
Anderson,RRox
中科院分区：
文献类型：
--
作者：
Yaroslavsky,AnnaN;Barbosa,Jose;Neel,Victor;DiMarzio,Charles;Anderson,RRox
Multispectral polarized light imaging (MSPLI) enables rapid inspection of a superficial tissue layer over large surfaces, but does not provide information on cellular microstructure. Confocal microscopy (CM) allows imaging within turbid media with resolution comparable to that of histology, but suffers from a small field of view. In practice, pathologists use microscopes at low and high power to view tumor margins and cell features, respectively. Therefore, we study the combination of CM and MSPLI for demarcation of nonmelanoma skin cancers. Freshly excised thick skin samples with nonmelanoma cancers are rapidly stained with either toluidine or methylene blue dyes, rinsed in acetic acid, and imaged using MSPLI and CM. MSPLI is performed at 630, 660, and 750 nm. The same specimens are imaged by reflectance CM at 630, 660, and 830 nm. Results indicate that CM and MSPLI images are in good correlation with histopathology. Cytological features are identified by CM, and tumor margins are delineated by MSPLI. A combination of MSPLI and CM appears to be complementary. This combinedin situtechnique has potential to guide cancer surgery more rapidly and at lower cost than conventional histopathology. ©2005 Society of Photo-Optical Instrumentation Engineers.
登录
查看更多内容
检测浸润前癌细胞现在可以原位发现癌前上皮细胞的预警变化
DOI：
--
发表时间：
--
期刊：
影响因子：
--
作者：
H. Overmars;John Jones;Vivian Blok;M. Wallace;L. Perelman;J. Arendt;R. Gurjar;M. G. Müller;Q. Zhang;G. Zonios;E. Kline;T. McGillican;S. Shapshay;J. Crawford;M. Fitzmaurice;S. Kabani;H. Levin;R. Dasari;I. Itzkan;J. Dam
通讯作者：
J. Dam
影响因子：
6.5
作者：
Rajadhyaksha, M;Menaker, G;González, S
通讯作者：
González, S
莫氏手术：基础知识和技术
DOI：
--
发表时间：
1998
期刊：
影响因子：
--
作者：
K. Gross;H. Steinman;R. Rapini
通讯作者：
R. Rapini
影响因子：
9.8
作者：
Drezek, RA;Collier, T;Follen, M
通讯作者：
Follen, M