Circulating tumour cells detected by a novel adenovirus-mediated system may be a potent therapeutic marker in gynaecological cancers.
Circulating tumour cells detected by a novel adenovirus-mediated system may be a potent therapeutic marker in gynaecological cancers.
复制标题
DOI：
10.1038/bjc.2012.276
复制
发表时间：
2012-07-24
影响因子：
8.8
通讯作者：
Inoue, M.
中科院分区：
文献类型：
--
作者：
Takakura, M.;Kyo, S.;Nakamura, M.;Maida, Y.;Mizumoto, Y.;Bono, Y.;Zhang, X.;Hashimoto, Y.;Urata, Y.;Fujiwara, T.;Inoue, M.
最近开发的循环肿瘤细胞(CTCs)检测系统使用端粒酶特异性复制腺病毒产生非特异性绿色荧光蛋白(GFP)信号，因为非特异性感染病毒的白细胞(WBC)共存。在这里，我们建立了一种独特的CTCs检测系统，完全排除了非特异性信号。从患者身上获得的血液经过溶血过程以消除红细胞。然后用OBP-401感染细胞球，固定，与荧光标记的抗CD45抗体孵育以标记白血WBC，并在显微镜下观察玻片。对人工添加到健康捐赠者样本中的癌细胞进行的预备实验证实，CD45标记可以区分GFP阳性的癌细胞和WBC。53例妇科肿瘤中，剔除GFP阳性细胞中的WBC后，21例(39.6%)检出CTCs。在健康志愿者的样本中未检测到CTCs。CTC计数与已知的临床病理因素无明显相关性。在大多数治疗有效的患者中，CTC在手术或化疗后迅速消失。相反，即使在治疗后，CTCs的持久性与治疗的不良反应密切相关(P<；0.005)。CTCs在我们的系统中的存在可能成为妇科癌症的一个新的治疗标志物。
Recently developed detection system for circulating tumour cells (CTCs) using a telomerase-specific replicative adenovirus generated nonspecific green fluorescent protein (GFP) signals because of the co-presence of white blood cells (WBCs) nonspecifically infected by viruses. Here, we established a unique detection system for CTCs that completely excludes nonspecific signals. Blood obtained from the patients was subjected to haemolytic processes to eliminate red blood cells. The cell pellets were then infected with OBP-401, fixed, incubated with fluorescence-labelled anti-CD45 antibody to mark white blood WBCs, and examined on slides under a microscope. Preparatory experiments with cancer cells artificially added to healthy donor samples confirmed that CD45 labelling could distinguish GFP-positive cancer cells from WBCs. In 53 patients with gynaecological cancers, CTCs were detected in 21 patients (39.6%) when CD45-positive cells were excluded as WBCs among GFP-positive cells. No CTCs were detected in samples from healthy volunteers. There was no significant correlation between CTC counts and known clinicopathological factors. The CTCs rapidly vanished after surgery or chemotherapy in most patients whose treatments were effective. In contrast, the persistence of CTCs even after treatments was tightly associated with poor response to the treatments (P<0.005). The presence of CTCs in our system may potentially be a novel therapeutic marker in gynaecological cancers.
登录
查看更多内容
影响因子：
45.3
作者：
Cristofanilli, M;Hayes, DF;Terstappen, LWMM
通讯作者：
Terstappen, LWMM
影响因子：
158.5
作者：
Cristofanilli, M;Budd, GT;Hayes, DF
通讯作者：
Hayes, DF
影响因子：
6.4
作者：
He, Wei;Kularatne, Surnith A.;Kalli, Kimberly R.;Prendergast, Franklyn G.;Amato, Robert J.;Klee, George G.;Hartmann, Lynn C.;Low, Philip S.
通讯作者：
Low, Philip S.
影响因子：
6.4
作者：
Fujiwara, Toshiya;Kagawa, Shunsuke;Fujiwara, Toshiyoshi
通讯作者：
Fujiwara, Toshiyoshi
影响因子：
3.8
作者：
Kim, Seung Jin;Masago, Akinori;Noguchi, Shinzaburo
通讯作者：
Noguchi, Shinzaburo