Surface Plasmon Resonance Imaging of Polymer Microarrays to Study Protein-Polymer Interactions in High Throughput
Surface Plasmon Resonance Imaging of Polymer Microarrays to Study Protein-Polymer Interactions in High Throughput
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DOI：
10.1021/la900735n
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发表时间：
2009-08-18
期刊：
影响因子：
3.9
通讯作者：
Voelcker, Nicolas H.
中科院分区：
文献类型：
--
作者：
Hook, Andrew L.;Thissen, Helmut;Voelcker, Nicolas H.
聚合物微阵列提供了一种高通量的格式，其中评估生物界面相互作用。这一奋进极大地帮助了先进生物材料的发展。为了增加该平台技术的范围，需要开发与微阵列格式兼容并且能够以高通量分析生物分子相互作用的分析工具。在这里，我们表明，表面等离子体共振成像（SPRI）是这样一个工具。SPRi能够并行地对多个表面生物分子相互作用进行空间分辨、表面敏感、无标记的实时分析。为了证明这一点，我们首先将苯基叠氮改性的聚合物印刷到其上，用低污垢基础聚合物涂布载玻片。载玻片的UV照射导致印刷的聚合物点与表面交联。利用SPRI对牛血清白蛋白的吸附和解吸进行了研究。胶原蛋白和纤连蛋白粘附在这些微阵列点上。还将斑点与所有粘附细胞系一起孵育，使得能够深入了解所研究的聚合物的细胞粘附的潜在机制。对于这里分析的系统，静电相互作用被证明占主导地位的细胞附着。
Polymer microarrays provide a high-throughput format in which to assess biointerfacial interactions. This endeavor greatly assists with the development of advanced biomaterials. In order to increase the scope of this platform technology, the development of analytical tools that are compatible with the microarray format and are capable of analyzing biomolecular interactions in high throughput is needed. Here, we show that surface plasmon resonance imaging (SPRi) is such a tool. SPRi enables spatially resolved, surface sensitive, label free, real-time analysis of multiple surfacebiomolecular interactions in parallel. In order to demonstrate this, we first printed phenylazide-modified polymers onto it slide coated with a low fouling base polymer. UV irradiation of the slide resulted in the cross-linking of the printed polymer spots to the Surface. SPRi was then employed to study the adsorption and desorption of bovine serum albumin. collagen, and fibronectin to these adhesive microarray spots. The spots were also incubated With all adherent cell line, enabling insight into the underlying mechanisms of cell attachment it) the polymers studied. For the system analyzed here, electrostatic interactions were shown to dominate cell attachment.