CORRELATION BETWEEN MORPHOLOGY AND FUNCTION IN ISOLATED ISLETS OF THE ZUCKER RAT
CORRELATION BETWEEN MORPHOLOGY AND FUNCTION IN ISOLATED ISLETS OF THE ZUCKER RAT
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DOI：
10.2337/diabetes.28.6.565
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发表时间：
1979-01-01
期刊：
影响因子：
7.7
通讯作者：
WOODSIDE, W
中科院分区：
文献类型：
--
作者：
HAYEK, A;WOODSIDE, W
Zucker大鼠的肥胖伴有高脂血症、高胰岛素血症、胰岛素抵抗、胰腺增生和胰岛肥大。这项研究将离体胰岛的形态异质性与灌注系统中胰岛素和胰高血糖素的分泌相关联。胰岛尺寸任意定义为大（> 0.45 mm）或小（< 0.12 mm）。分别计算组和单个胰岛的蛋白质含量和体积(V = 4/3.pi.r3)。来自肥胖大鼠的胰岛对葡萄糖和氨茶碱的反应比来自瘦大鼠的胰岛分泌更多的胰岛素（峰值7.8.+-.2.4对比1.5.+-.0.37μ单位/胰岛每分钟，P<0.005）。胰岛素释放与胰岛大小和蛋白质含量直接相关。来自瘦和肥胖动物的小胰岛每个胰岛产生的胰岛素少于大胰岛（P < 0.005）。然而，就胰岛体积而言，与小胰岛相比，大胰岛的胰岛素释放效率较低（P < 0.005）。用Br-c[环]AMP刺激从瘦动物的胰岛释放胰高血糖素，但不从肥胖动物的大胰岛释放胰高血糖素（峰值11.+-.3.3与4.1.+-.0.3pg/.mu.g蛋白质每分钟，P<0.05）。 Arg 对胰高血糖素释放产生与 Br-cAMP 刺激相同的效果 (P < 0.05)。观察到的胰岛素释放率增加和胰高血糖素反应减弱与 Zucker 大鼠胰腺中的胰岛大小有关。
Obesity in the Zucker rat is accompanied by hyperlipemia, hyperinsulinism, insulin resistance, pancreatic hyperplasia, and islet hypertrophy. This study correlates the morphologic heterogeneity of isolated pancreatic islets with secretion of insulin and glucagon in a perifusion system. Islet size was arbitrarily defined as large (> 0.45 mm) or small (< 0.12 mm). Protein content and volume (V = 4/3 .pi.r3) were calculated for groups and individual islets, respectively. Islets from obese rats secreted more insulin in response to glucose and aminophylline than islets from lean rats (peak 7.8 .+-. 2.4 vs. 1.5 .+-. 0.37 .mu.units/islet per min, P < 0.005). Insulin release was related directly to islet size and protein content. Small islets from lean and obese animals produced less insulin per islet than large islets (P < 0.005). In terms of islet volume, however, large islets were inefficient insulin releasers as compared to small islets (P < 0.005). Stimulation with Br-c[cyclic]AMP released glucagon from islets of lean but not from large islets of obese animals (peak 11 .+-. 3.3 vs. 4.1 .+-. 0.3 pg/.mu.g protein per min, P < 0.05). Arg produced the same effect on glucagon release (P < 0.05) as stimulation with Br-cAMP. The observed increased insulin release rates and the blunted glucagon response are related to islet size in the pancreas of the Zucker rat.