EVIDENCE FOR KERATIN PROTEINS IN NORMAL AND ABNORMAL HUMAN MEIBOMIAN FLUIDS
EVIDENCE FOR KERATIN PROTEINS IN NORMAL AND ABNORMAL HUMAN MEIBOMIAN FLUIDS
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DOI：
10.3109/02713689109024128
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发表时间：
1991-12-01
影响因子：
2
通讯作者：
LARKE, JR
中科院分区：
文献类型：
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作者：
ONG, BL;HODSON, SA;LARKE, JR
睑板腺过度角化被认为会导致腺体功能障碍。最近对兔子的研究表明，角蛋白确实存在于这些动物的睑板液中。在本报告中，我们介绍了关于人类睑板分泌物中这些不溶于水的蛋白质的存在的研究结果。使用 6 种抗细胞角蛋白抗体 CK8、18、19、CK7、CK8、CK14、CK19 和 AE1/AE3 来对抗人睑板液表达的角蛋白。使用免疫印迹（斑点印迹）技术，将从诊断为睑板腺功能障碍（MGD）的受试者获得的异常蜡质睑板液与正常透明睑板液进行比较。结果表明，与正常人相比，异常人类睑板分泌物中角蛋白的浓度更高（10%）。尽管异常睑板排泄物中未显示角化蛋白标记物的存在，但异常睑板液中角蛋白蛋白的增加表明，在 MGD 患者中，导管上皮可能已发生过度角化。除了导管上皮通常产生的角蛋白之外，还产生了更多的角蛋白（可能是较高分子量的角蛋白）。正如其他研究人员所假设的，异常睑板液中角蛋白数量的增加可能是由于导管上皮对过度角化过程的敏感性所致。
Hyperkeratinization of meibomian glands has been postulated to cause gland dysfunction. Recent investigations on rabbits show that keratin proteins are indeed present in the meibomian fluids of these animals. In this report we present our findings on the presence of these water-insoluble proteins in human meibomian secretions. 6 anti-cytokeratin antibodies, CK8, 18, 19, CK7, CK8, CK14, CK19 and AE1/AE3 were used against the keratin proteins expressed from the human meibomian fluids. Using the immunoblotting (dot blot) technique, abnormal waxy meibomian fluids obtained from subjects diagnosed to have meibomian gland dysfunction (MGD) were compared to normal clear meibomian fluids. The results show that keratins are present in a higher concentration (10%) in the abnormal human meibomian excreta as compared to the normals. Even though the presence of protein markers for keratinization in the abnormal meibomian excreta were not shown, the increased presence of keratin proteins in the abnormal meibomian fluids suggests that, in MGD patients, hyperkeratinization of ductal epithelium may have taken place. More keratin proteins (possibly those of higher molecular weights) were produced in addition to the keratin proteins normally produced by the duct epithelium. The increased amount of keratin proteins in the abnormal meibomian fluids may be explained by the susceptibility of duct epithelium to undergo the process of hyperkeratinization as postulated by other researchers.