Contraction of collagen-glycosaminoglycan matrices by peripheral nerve cells in vitro
Contraction of collagen-glycosaminoglycan matrices by peripheral nerve cells in vitro
复制标题
DOI：
10.1016/s0142-9612(00)00345-8
复制
发表时间：
2001-05-01
期刊：
影响因子：
14
通讯作者：
Spector, M
中科院分区：
文献类型：
--
作者：
Spilker, MH;Asano, K;Spector, M
本研究的目的是在体外研究胶原-糖胺聚糖 (GAG) 基质中周围神经支持细胞的收缩行为。已知收缩性成纤维细胞（肌成纤维细胞）在选定的结缔组织（即真皮）愈合过程中参与伤口收缩，但对周围神经愈合过程中非肌肉收缩细胞的活性知之甚少。将成年大鼠坐骨神经的外植体置于胶原-GAG 基质盘上并在培养物中维持长达 30 天。对平均孔径和交联度不同的胶原-GAG 基质组进行了测试。检查从神经外植体到基质的细胞迁移，并使用免疫组织化学染色来鉴定表达收缩肌动蛋白亚型（α-平滑肌肌动蛋白；α-SMA）的细胞和雪旺细胞（S-100）。每五天将矩阵盘的几何收缩量化为盘直径的减少百分比。基质盘的收缩量受交联程度的显着影响。细胞迁移到基质中以及α-SMA或S-100染色的细胞分布不受基质参数的影响。这些研究表明，来自周围神经外植体的细胞能够采用收缩表型并在体外引起基质的几何收缩，并表明收缩过程在体内神经伤口愈合过程中可能很重要。 (C) 2001 Elsevier Science Ltd. 保留所有权利。
The objective of this study was to investigate the contractile behavior of peripheral nerve support cells in collagen-glycosaminoglycan (GAG) matrices in vitro. Contractile fibroblasts (myofibroblasts) are known to participate in wound contraction during healing of selected connective tissues (viz., dermis), but little is known about the activity of non-muscle contractile cells during healing of peripheral nerves. Explants from adult rat sciatic nerves were placed onto collagen-GAG matrix disks and maintained in culture for up to 30 days. Groups of collagen-GAG matrices were tested that differed in average pore diameter and in degree of cross-linking. Cell migration from nerve explants into the matrices was examined, and immunohistochemical staining was used to identify cells expressing a contractile actin isoform (alpha -smooth muscle actin; alpha -SMA) and Schwann cells (S-100). Geometric contraction of matrix disks was quantified every five days as the percent reduction in disk diameter. The amount of contraction of matrix disks was significantly affected by the degree of cross-linking. Cell migration into the matrices and the distribution of cells staining for alpha -SMA or S-100 was not affected by matrix parameters. These studies demonstrate that cells From peripheral nerve explants were capable of adopting a contractile phenotype and causing geometric contraction of matrices in vitro and suggest that contractile processes may be important during nerve wound healing in vivo. (C) 2001 Elsevier Science Ltd. All rights reserved.