Sex reversal in C57BL/6J-Y-POS mice corrected by a Sry transgene
Sex reversal in C57BL/6J-Y-POS mice corrected by a Sry transgene
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DOI：
10.1098/rstb.1995.0160
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发表时间：
1995-11-29
影响因子：
6.3
通讯作者：
Washburn, LL
中科院分区：
文献类型：
--
作者：
Eicher, EM;Shown, EP;Washburn, LL
携带家鼠 Y 染色体 (Y-POS) 的 C57BL/6J 小鼠发育为具有卵巢组织的雌性或具有卵巢和睾丸组织的雌雄同体。我们测试了这样的假设：这种遗传性性逆转的 Y 连锁部分是由 M. d 引起的。通过检查携带 a-M 的 C57BL/6J XY(POS) 小鼠的性腺发育，确定后斯基亚维努斯 Y 连锁睾丸决定基因（符号为 Tdy 或 Sry）。 Sry 的肌肉等位基因作为转基因。我们发现，在存在转基因的情况下，XY(POS) 小鼠只发育出睾丸组织。该结果表明，Y-POS染色体上携带的Sry等位基因负责C57BL/6J近交系背景中卵巢组织的发育。我们根据各种家蝇和小家鼠 Y 染色体携带的 Sry 等位基因中存在的 DNA 多态性讨论了这一发现。此外，我们基于卵睾中卵巢和睾丸组织的组织，提出了关于发育中胎儿性腺中睾丸和卵巢决定基因表达时间的假设。
C57BL/6J mice carrying a Mus domesticus poschiavinus Y chromosome (Y-POS) develop as females with ovarian tissue or as hermaphrodites with ovarian and testicular tissue. We tested the hypothesis that the Y-linked component of this inherited sex reversal is caused by the M. d. poschiavinus Y-linked testis determining gene (symbolized Tdy or Sry) by examining gonadal development in C57BL/6J XY(POS) mice carrying a-M. musculus allele of Sry as a transgene. We found that in the presence of the transgene, XY(POS) mice developed exclusively testicular tissue. This result indicates that the Sry allele carried on the Y-POS chromosome is responsible for development of ovarian tissue in the C57BL/6J inbred strain background. We discuss this finding in light of DNA polymorphisms present in Sry alleles carried by various M. domesticus and M. musculus Y chromosomes. In addition, we present a hypothesis concerning the timing of expression of the testicular and ovarian determining genes in the developing fetal gonad based on the organization of ovarian and testicular tissue in ovotestes.