Synthesis, characterization and electrochemistry studies of iron(III) complex with curcumin ligand
Synthesis, characterization and electrochemistry studies of iron(III) complex with curcumin ligand
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DOI：
10.1111/1440-1681.12964
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发表时间：
2018-06
影响因子：
2.9
通讯作者：
Gülüzar Özbolat;A. Yegani;A. Tuli
中科院分区：
文献类型：
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作者：
Gülüzar Özbolat;A. Yegani;A. Tuli
铁超载是人类的一种严重的临床症状，也是药物开发的关键靶点。本研究的目的是研究铁(III)离子与姜黄素配体的配位作用，以用于治疗铁超载。合成了姜黄素铁(III)配合物，并用FT-IR、UV-Vis、元素分析和磁化率等方法对其结构进行了表征。用循环伏安法研究了配体和配合物的电化学行为。用四甲基偶氮唑盐比色法测定配体和铁(III)络合物的细胞毒活性。在室温生理pH条件下，姜黄素与高浓度铁发生反应。随后，形成了棕红色的复合体。关于磁化率的数据表明，具有1：2(金属/配体)摩尔比的配合物具有八面体几何构型。该络合物对ECV304细胞具有较强的抗氧化作用，其IC50值为4.83，高于姜黄素。该络合物具有很高的细胞毒活性，其细胞毒作用远好于配体。氧化还原电位分别为0.180 V和0.350 V。电化学研究表明，Fe~(3+)/Fe~(2+)在低电位下发生氧化还原反应。这一数值在预计显示超氧化物歧化酶活性的化合物范围内。这一发现表明，铁复合体能够清除自由基。观察到的细胞毒性可以用来获得潜在的药物。需要进一步研究为此目的使用姜黄素的情况。
Iron overload is a serious clinical condition for humans and is a key target in drug development. The aim of this study was to investigate the coordination of iron(III) ions with curcumin ligand that may be used in the treatment of iron overload. Iron(III) complex of curcumin was synthesized and structurally characterized in its solid and solution state by FT‐IR, UV‐Vis, elemental analysis, and magnetic susceptibility. Electrochemical behaviour of the ligand and the complexes were examined using cyclic voltammetry. The cytotoxic activities of the ligand and the iron(III) complex were evaluated by the MTT assay. Curcumin reacted with iron in high concentrations at physiological pH at room temperature. Subsequently, a brown‐red complex was formed. Data regarding magnetic susceptibility showed that the complexes with a 1:2 (metal/ligand) mole ratio had octahedral geometry. The complex showed higher anti‐oxidant effect towards the cell line ECV304 at IC50 values of 4.83 compared to curcumin. The complex exhibited very high cytotoxic activity and showed a cytotoxic effect that was much better than that of the ligand. The potentials for redox were calculated as 0.180 V and 0.350 V, respectively. The electrochemistry studies showed that Fe3+/Fe2+ couple redox process occurred at low potentials. This value was within the range of compounds that are expected to show superoxide dismutase activity. This finding indicates that the iron complex is capable of removing free radicals. The observed cytotoxicity could be pursued to obtain a potential drug. Further studies investigating the use of curcumin for this purpose are needed.