In situ-forming robust chitosan-poly(ethylene glycol) hydrogels prepared by copper-free azide-alkyne click reaction for tissue engineering.
In situ-forming robust chitosan-poly(ethylene glycol) hydrogels prepared by copper-free azide-alkyne click reaction for tissue engineering.
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DOI：
10.1039/c3bm60159e
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发表时间：
2014-02
影响因子：
6.6
通讯作者：
V. Truong;Matthew P. Ablett;Hamish T. J. Gilbert;J. Bowen;S. Richardson;J. Hoyland;A. P. Dove
中科院分区：
文献类型：
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作者：
V. Truong;Matthew P. Ablett;Hamish T. J. Gilbert;J. Bowen;S. Richardson;J. Hoyland;A. P. Dove
水溶性叠氮官能化壳聚糖与丙炔酸酯官能化聚（乙二醇）使用无铜点击化学交联。所得的水凝胶材料在37 °C下在5-60分钟内形成，并产生具有可调性质如溶胀、机械强度和降解的机械坚固材料。重要的是，水凝胶支持间充质干细胞附着和增殖，并且对包封的细胞也是无毒的。因此，这些研究表明，水凝胶有潜力被用作组织工程的可注射生物材料。
A water-soluble azide-functionalised chitosan was crosslinked with propiolic acid ester-functional poly(ethylene glycol) using copper-free click chemistry. The resultant hydrogel materials were formed within 5-60 min at 37 °C and resulted in mechanically robust materials with tuneable properties such as swelling, mechanical strength and degradation. Importantly, the hydrogels supported mesenchymal stem cell attachment and proliferation and were also non-toxic to encapsulated cells. As such these studies indicate that the hydrogels have potential to be used as injectable biomaterials for tissue engineering.