Fates of Fe3O4 and Fe3O4@SiO2 nanoparticles in human mesenchymal stem cells assessed by synchrotron radiation-based techniques
Fates of Fe3O4 and Fe3O4@SiO2 nanoparticles in human mesenchymal stem cells assessed by synchrotron radiation-based techniques
复制标题
通过基于同步辐射的技术评估 Fe3O4 和 Fe3O4@SiO2 纳米粒子在人间充质干细胞中的命运
DOI：
10.1016/j.biomaterials.2014.04.052
复制
发表时间：
2014-08-01
期刊：
影响因子：
14
通讯作者：
Wang, Qiangbin
中科院分区：
文献类型：
--
作者：
Tian, Fei;Chen, Guangcun;Wang, Qiangbin
超顺磁性氧化铁纳米粒子（SPIO）作为磁共振成像（MRI）造影剂已广泛应用于生物医学研究和临床应用，最近在体内干细胞追踪方面特别受到关注。然而，SPIO在细胞中的命运尚未完全了解，这对于干细胞特别重要，因为微环境的任何变化都可能干扰其增殖和分化行为。本文采用同步辐射X射线荧光光谱（XRF）结合X射线吸收光谱（XAS）技术，原位研究了Fe3O4和Fe3O4@SiO2纳米颗粒在人骨髓间充质干细胞（hMSCs）中的分布和化学形态的动态变化。XAS分析表明，Fe3O4 NPs与hMSC一起培养是相当稳定的，并且几乎保持其初始化学形式长达14天，这与先前的报道相矛盾，即Fe3O4 NPs在使用简化的溶酶体模型系统评估的细胞标记中是不稳定的。用SiO2壳包覆，Fe3O4@SiO2 NPs在hMSC中表现出更高的稳定性，而其化学形式没有可检测的变化。此外，XRF分析表明，Fe3O4@SiO2 NPs可以以高效的方式标记hMSCs，并且在细胞增殖期间仅分布在细胞质中，使其成为体内干细胞追踪的理想探针。在基于同步辐射的XAS和XRF的帮助下，这些发现提高了我们对给予hMSC的SPIO的命运的理解，并将有助于SPIO的未来设计，以实现安全有效的干细胞跟踪。(C)2014爱思唯尔有限公司版权所有。
Superparamagnetic iron oxide nanoparticles (SPIOs) have been widely used as the magnetic resonance imaging (MRI) contrast agent in biomedical studies and clinical applications, with special interest recently in in vivo stem cell tracking. However, a full understanding of the fate of SPIOs in cells has not been achieved yet, which is particularly important for stem cells since any change of the microenvironment may disturb their propagation and differentiation behaviors. Herein, synchrotron radiation-based X-ray fluorescence (XRF) in combination with X-ray absorption spectroscopy (XAS) were used to in situ reveal the fate of Fe3O4 and Fe3O4@SiO2 NPs in human mesenchymal stem cells (hMSCs), in which the dynamic changes of their distribution and chemical speciation were precisely determined. The XAS analysis evidences that Fe3O4 NPs cultured with hMSCs are quite stable and almost keep their initial chemical form up to 14 days, which is contradictory to the previous report that Fe3O4 NPs were unstable in cell labeling assessed by using a simplified lysosomal model system. Coating with a SiO2 shell, Fe3O4@SiO2 NPs present higher stability in hMSCs without detectable changes of their chemical form. In addition, XRF analysis demonstrates that Fe3O4@SiO2 NPs can label hMSCs in a high efficiency manner and are solely distributed in cytoplasm during cell proliferation, making it an ideal probe for in vivo stem cell tracking. These findings with the help of synchrotron radiation-based XAS and XRF improve our understanding of the fate of SPIOs administered to hMSCs and will help the future design of SPIOs for safe and efficient stem cells tracking. (C) 2014 Elsevier Ltd. All rights reserved.