Characterization of zebrafish PSD-l95 gene family members
Characterization of zebrafish PSD-l95 gene family members
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DOI：
10.1002/neu.20118
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发表时间：
2005-05-01
期刊：
影响因子：
--
通讯作者：
Smith, SJ
中科院分区：
文献类型：
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作者：
Meyer, MP;Trimmer, JS;Smith, SJ
膜相关鸟苷酸激酶（MAGUKs）的PSD-95家族被认为充当调节在兴奋性突触的突触后密度处发现的多蛋白信号传导复合物的组装和功能的分子支架。迄今为止，哺乳动物神经系统中PSD-95家族成员的遗传分析是困难的，但斑马鱼正在成为研究特定基因在发育和成熟神经系统中的作用的理想脊椎动物系统。在这里，我们描述了斑马鱼的PSD-95，PSD-93，和SAP-97的两种亚型的直系同源物的克隆。使用原位杂交分析，我们发现，这些斑马鱼MAGUKs有重叠，但不同的模式在发育中的神经系统和颅面骨骼的表达。使用泛MAGUK抗体，我们表明，MAGUK蛋白定位于神经元内的发展后脑，小脑，视觉和嗅觉系统，以及皮肤上皮细胞。在嗅觉和视觉系统中，MAGUK蛋白在突触区域中强烈表达，并且在这些区域中表达的开始与突触形成的时期一致。这些数据与PSD-95家族成员参与突触组装和功能的想法一致，并为将来在高度易处理的模型生物体中进行体内功能研究提供了平台。(c)2005 Wiley Periodicals，Inc.
The PSD-95 family of membrane-associated guanylate kinases (MAGUKs) are thought to act as molecular scaffolds that regulate the assembly and function of the multiprotein signaling complex found at the postsynaptic density of excitatory synapses. Genetic analysis of PSD-95 family members in the mammalian nervous system has so far been difficult, but the zebrafish is emerging as an ideal vertebrate system for studying the role of particular genes in the developing and mature nervous system. Here we describe the cloning of the zebrafish orthologs of PSD-95, PSD-93, and two isoforms of SAP-97. Using in situ hybridization analysis we show that these zebrafish MAGUKs have overlapping but distinct patterns of expression in the developing nervous system and craniofacial skeleton. Using a pan-MAGUK antibody we show that MAGUK proteins localize to neurons within the developing hindbrain, cerebellum, visual and olfactory systems, and to skin epithelial cells. In the olfactory and visual systems MAGUK proteins are expressed strongly in synaptic regions, and the onset of expression in these areas coincides with periods of synapse formation. These data are consistent with the idea that PSD-95 family members are involved in synapse assembly and function, and provide a platform for future functional studies in vivo in a highly tractable model organism. (c) 2005 Wiley Periodicals, Inc.