miR-194 as a Predictor for Adenoma Recurrence in Patients with Advanced Colorectal Adenoma after Polypectomy
miR-194 as a Predictor for Adenoma Recurrence in Patients with Advanced Colorectal Adenoma after Polypectomy
复制标题
DOI：
10.1158/1940-6207.capr-13-0426
复制
发表时间：
2014-06-01
影响因子：
3.3
通讯作者：
Fang, Jing-Yuan
中科院分区：
文献类型：
--
作者：
Wang, Zhen-Hua;Ren, Lin-Lin;Fang, Jing-Yuan
MicroRNAs(MiRNA)是一种很有前途的结直肠癌(CRC)预测因子。我们研究了miRNAs是否可以预测晚期结直肠腺瘤(ACRA)息肉切除术后的腺瘤复发。用miRNA微阵列进行miRNA表达谱分析，以确定与复发相关的miRNAs。从ACRA患者的福尔马林固定石蜡包埋块中提取候选miRNAs，用实时荧光定量聚合酶链式反应(Real-time PCR)检测。Logistic回归分析用来调查有效的miRNA表达谱是否独立于其他已知的腺瘤复发危险因素。通过受试者工作特征(ROC)曲线下面积分析评估6个miRNAs和3个独立危险因素的预后价值。在这组回顾性队列研究中，6个候选miRNAs在正常结直肠组织中的表达显著低于腺瘤复发的ARCA(RACRA)。然而，只有miRNA(MiR)-194成为一个实用的预测因子。在回溯性队列中，以0.1311为临界值，MIR-194作为预测指标的敏感性和特异性分别为71.0%和78.0%。在使用相同截断值的前瞻性队列中，灵敏度和特异度分别为76.1%和77.2%。MiR-194低表达、腺瘤大小<2 cm、腺瘤<3>是腺瘤复发的独立危险因素。此外，ROC曲线分析显示，miR-194的低表达对腺瘤复发的预测优于腺瘤的大小和数目。MIR-194可能是ACRA息肉切除术后腺瘤复发的独立预测因子。(C)2014年AACR。
microRNAs (miRNA) are promising predictors in colorectal cancer (CRC). We investigated whether miRNAs could predict adenoma recurrence in patients with advanced colorectal adenoma (ACRA) after polypectomy. miRNA expression profiling was performed by miRNA microarray to identify recurrence-related miRNAs. Candidate miRNAs extracted from formalin-fixed paraffin-embedded blocks of patients with ACRA were measured using real-time PCR. Logistic regression analysis was conducted to investigate whether validated miRNA expression profiles were independent from other known adenoma recurrence risk factors. The prognostic values of six miRNAs and three independent risk factors were assessed by the area under the receiver operating characteristic (ROC) curve analysis. The expressions of six candidate miRNAs were significantly decreased from levels in normal colorectal tissue compared with ARCA with adenoma recurrence (RACRA) in this retrospective cohort. However, only miRNA (miR)-194 emerged as a practical predictor. The sensitivity and specificity of miR-194 as a predictor were 71.0% and 78.0%, respectively, at a cutoff value of 0.1311 in the retrospective cohort. Sensitivity and specificity were 76.1% and 77.2%, respectively, in the prospective cohort using the same cutoff value. Low expression levels of miR-194, adenoma size >2 cm, and >3 adenomas were independent risk factors for adenoma recurrence. Moreover, low expression of miR-194 was a better predictor of adenoma recurrence than the adenoma size and numbers according to ROC curve analysis. miR-194 may be an independent predictor for adenoma recurrence in patients with ACRA after polypectomy. (C)2014 AACR.