Production of 2-phenylethanol in hybrid system using airlift reactor and immersed hollow fiber membrane module
Production of 2-phenylethanol in hybrid system using airlift reactor and immersed hollow fiber membrane module
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DOI：
10.1016/j.cep.2013.08.008
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发表时间：
2013-10-01
影响因子：
4.3
通讯作者：
Markos, Jozef
中科院分区：
文献类型：
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作者：
Mihal', Mario;Kristofikova, Ludmila;Markos, Jozef
在许多化学专业的生物生产中，为了最大限度地降低资本和操作成本，其中产品抑制生物反应，基于膜萃取应用的混合系统集成在生物反应器中去除产品是一个合适的解决方案。集成可以通过外部膜提取模块来完成，或者作为一种更有效的解决方案，通过直接浸入生物反应器中的提取膜模块来完成。在第二种情况下，没有必要使用微过滤来防止膜污染，也没有必要在膜模块中使用另一个泵进行壳流。此外，该系统结构紧凑、高效、抗故障，并可进行数学模拟。这些陈述在本文中得到了证明，其中一个混合系统由气升反应器和浸入式提取中空纤维膜模块组成，用于酵母将l -苯丙氨酸生物转化为所需的玫瑰状香气，2-苯乙醇。采用不同的饲养和曝气策略进行了两项生物转化试验。在这两个实验中，l -苯丙氨酸的高转化率（高达100%）和2-苯乙醇的高体积产量（高达18.6 g L-1）都达到了。这两个生物转化实验在数学上进行了预测，实验数据和模拟结果吻合良好。(C) 2013 Elsevier B.V.版权所有
To minimize capital and operative costs in many bioproductions of chemical specialities, where the product inhibits the bioreaction, a hybrid system based on the application of membrane extraction integrated in the bioreactor to remove the product is a suitable solution. Integration can be done by an external module for membrane extraction or, as a more effective solution, by an extraction membrane module immersed directly in the bioreactor. In this second case, it is not necessary to use microfiltration to prevent membrane fouling or to use another pump for shell flow in the membrane module. Moreover, the system is very compact, highly effective, resistant to failures and its mathematic simulation is also possible. These statements are proved in this paper where a hybrid system consisting of an airlift reactor and immersed extraction hollow fiber membrane module was used for the biotransformation of L-phenylalanine to the desired rose-like aroma, 2-phenylethanol, by yeasts Saccharomyces cerevisiae. Two biotransformation experiments were carried out using different feeding and aeration strategies. In both experiments, high conversion of L-phenylalanine (up 100%) and high volumetric production of 2-phenylethanol (up 18.6 g L-1) were reached. Both biotransformation experiments were mathematically predicted with good agreement between experimental data and simulations. (C) 2013 Elsevier B.V. All rights reserved.